大鼠睾丸支持细胞雄激素结合蛋白mRNA在生精周期中的表达  

Stage-dependent Expression of Androgen Binding Protein mRNA in Sertoli Cell of Rat Testis

作　　者：陈咏梅[1] 叶世隽[1] 黄玉苓 郑文利 

机构地区：[1]中国医学科学院中国协和医科大学基础医学研究所组织胚胎教研室,北京100005

出　　处：《中国医学科学院学报》2000年第3期287-289,共3页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学基金! (39770 778)

摘　　要：目的　研究大鼠睾丸支持细胞 (sertoli cell,SC)雄激素结合蛋白 (ABP) m RNA水平与曲细精管上皮周期的相关关系。方法　以地高辛标记的大鼠 ABP c DNA探针在 SD大鼠睾丸冰冻切片上进行原位杂交 ,应用激光密度扫描系统对阳性信号进行定量。结果　 ABP m RNA在 - 期维持低水平 ; - 期迅速升高 ,达到高峰 ; - 期骤然下降 ,但仍高于 - 期水平 , - 期又略有回升 ;随后迅速下降至 - 期水平。结论　 SC内 ABPm RNA水平具有明显的生精周期依赖性变化。To investigate the variation of rat androgen binding protein(ABP)mRNA in sertoli cell of rat testis during spermatogenesis. Methods In situ hybridization was performed with digoxin-labeled rat ABP cDNA probe on frozen testis tissue sections from SD rats. The expression of ABP mRNA was quantified by using laser density scanning system. Results It was shown that ABP mRNA levels were low from stage Ⅰ to stage Ⅵ,increased to the peak level at the stages Ⅶ-Ⅷ,and then declined rapidly at stages Ⅸ-Ⅺ.Thereafter,ABP mRNA levels increased again at stages ⅩⅡ-ⅩⅣ,and then decreased to the lowest level at stages Ⅰ-Ⅵ. Conclusions ABP mRNA level was stage-dependent during the spermatogenic cycle.

关 键 词：支持细胞 雄激素结合蛋白 睾丸 MRNA 生精周期 

分 类 号：R322.64[医药卫生—人体解剖和组织胚胎学]