^(99m)锝标记紫杉醇脂质体在移植荷瘤鼠体内分布的研究  

Study on the Biodistribution of^(99m) TC-labeled Paclitaxel Liposome in Xenograft bearing Nude Mice

作　　者：张长英[1] 成文彩[1] 王央贡[2] 崔武任[2] 赵明[3] 朱晓华 

机构地区：[1]同济医科大学附属同济医院妇产科,武汉430030 [2]同济医科大学实验医学研究中心核医学教研室,武汉430030 [3]同济医科大学附属同济医院同位素室,武汉430030

出　　处：《同济医科大学学报》2000年第3期253-255,共3页Acta Universitatis Medicinae Tongji

摘　　要：对 2 0只雌性裸鼠建立人卵巢癌裸鼠皮下移植瘤模型 ,经其尾静脉注射锝标紫杉醇脂质体 (99m TC- TL) 0 .2m l,于 15 min、 30 m in、 90 m in各处死动物 5只 ,将心、肝、脾、肺、肾、小肠、子宫附件、骨及肿瘤组织分别称重。其余 5只动物尾静脉注射游离锝 (99m TC— F) 0 .2 m l,于 90 min处死 ,取肝、脾、肺及肿瘤组织称重 ,用 γ-定标仪测其放射强度 (单位 :CPM/ 10 0 mg)。肝、脾、肺脏器内 99m TC动态放射值最高 (5 5 2 .1± 92 .8～ 2 6 0 .1± 2 1.0 cpm/ 10 0m g) ,其放射强度随时间延长呈下降趋势 ,肿瘤组织放射值较低 (3.6± 0 .6 cpm/ 10 0 m g) ,动态测量呈水平位。99m TC- TL组与 99m TC- F组在 99m TC示踪显示 90 min时 ,TC- TL 组肝、脾、肺内放射强度高于 99m TC- F组 (P<0 .0 5 ) ,而肿瘤组织中两组无显著性差异 (P>0 .0 5 )。结论 :静注紫杉醇脂质体在荷瘤鼠体内主要定向分布于网状内皮系统丰富的肝、脾、肺器官内 ,肿瘤组织有一定滞留性 。Subcutaneous xenograft tumor model was established in 20 female nude mice. 99m TC paclitaxel liposome ( 99m TC TL) 0.2 ml was injected through tail vein into the mice. Five animals were killed in each time point of 15 min, 30 min, 90 min after injection. The major organs were taken out and accurately weighed including heart, liver, spleen, lung, kidney, intestine, uterus and appendix, skeleton and tumor tissue. The remaining 5 mice were subjected to the injection of 0.2 ml 99m TC solution and killed 90 min after injection. Liver, spleen, lung and tumor tissue were taken out and weighed. γ measure device was used to measure the tissue radioactive intensity. Radioactive intensity was the highest(552 1±92 8 to 260 1±21 0 CPM/100 mg tissue)in spleen, liver and lung relatively, and tended to decrease with the prolongation of the time. The intensity in tumor tissue was obviously lower(3 6±0 6 CPM/100 mg tissue), but kept relative stabilization and did not show a decreasing tendency. It was found that there was statistically significant difference in radioactive intensity in liver, spleen and lung tissues ( P <0 05), but there was no significant difference in tumor tissue ( P >0 05) between 99m TC TL group and 99m TC solution group. It was concluded that paclitaxel liposome after intravenous injection was mostly accumulated in liver, spleen, lung tissues in xenograft bearing mice and showed a target ability. There was certain intensity in tumor tissue but it did not show target ability as compared with 99m TC solution.

关 键 词：脂质体 紫杉醇 抗癌药 移植荷瘤 体内分布 

分 类 号：R979.1[医药卫生—药品] R73－354[医药卫生—药学]