钳夹型急性大鼠脊髓损伤模型的建立与评价  

Establishment and Evaluation of Rat Clip Compression Model for Spinal Cord Injury

作　　者：李经辉[1] 黄辉[1] 吴海鹰[2] 张云茜[3] 熊飞[1] 余化霖[1] 

机构地区：[1]昆明医科大学第一附属医院微创神经外科,云南昆明650032 [2]昆明医科大学第一附属医院急诊科,云南昆明650032 [3]云南省第二人民医院神经内科,云南昆明650021

出　　处：《昆明理工大学学报（自然科学版）》2012年第6期67-71,75,共6页Journal of Kunming University of Science and Technology(Natural Science)

基　　金：云南省联合基金(2008CD006);云南省教育厅基金(2011y184);昆明医科大学博士研究生创新基金(2012D06)

摘　　要：建立一种大鼠的急性脊髓钝性损伤模型并对其进行评估.随机将64只SD大鼠分成脊髓损伤组与对照组.脊髓损伤组建立脊髓T10节段垂直钳夹脊髓损伤模型;对照组打开椎管,不伤脊髓;建模后1 d、3 d、5 d、7 d、14 d、21 d、28 d分别进行BBB行为学评分,体感诱发电位检测,评估神经传导通路的完整性,取局部受损脊髓病理检查.与对照组相比,脊髓损伤组术后1 d、3 d、5d、7 d、14 d、21 d、28 d BBB评分均降低(P<0.05),随时间延长BBB评分逐渐增高;术后6 h、1d、3 d、5 d、7 d未检测出体感诱发电位,14 d后均能检测出;同期病理观察显示脊髓损伤组大鼠脊髓钝性损伤后局部变性,充血,水肿,空洞形成.所以,钳夹型急性大鼠脊髓损伤模型是一种能很好模拟临床SCI的模型,操作简单,易于重复,其实验指标稳定,适合于脊髓损伤基础研究.A rat model of acute compression spinal cord injuries is established.Relevant evaluations are then carried out.64 SD rats are divided randomly into a spinal cord injury group and a control group.T10 segmental vertical compressing model is set up in the spinal cord injury group.The spinal canals are opened in the control group,without any hurt to the spinal cord.After modeling 1d,3d,5d,7d,14d,21d and 28d,BBB ethologic scores,the SEPs detection,the pathological examination of the damaged spinal cord are performed respectively.Compared with the control group,BBB scores of spinal cord injury group after surgery at 1d,3d,5d,7d,14d,21d and 28d are all lower（P0.05）.In the modeling group,with the extension of time its score is higher.Not being evoked out in the model group at 6h,1d,3d and 7d after modeling,the SEP can be detected in all the subjects out after 14 days.In the meantime,morphological observation results show that its local degeneration,congestion,edema,empty formation are observed.Therefore,with its stability and its easiness to operate and repeat,this model simulates well the clinical SCI,which is suitable for basic research of SCI.

关 键 词：大鼠脊髓损伤 病理改变 体感诱发电位 

分 类 号：R651.2[医药卫生—外科学]