Indication of Genetic Linkage Map for Sunflower(Helianthus annuus L.) by SSR Markers  

SSR分子标记丰富向日葵(Helianthus annuus L.)遗传图谱的研究(英文)

作　　者：黄先群[1] Genzbitelle L. Fabre F. Saraffi A. 

机构地区：[1]贵州省生物技术研究所,贵州省农业生物技术重点实验室,贵州贵阳550006 [2]法国图鲁兹国立综合研究院生物技术和作物改良实验室,INP-ENSAT,Pole de Biotechnologie Végétale

出　　处：《Agricultural Science & Technology》2012年第12期2489-2495,2495,共7页农业科学与技术（英文版）

基　　金：Supported by the Spring Sunshine Plan of PRC Ministry of Education for Scholars Studied in France,office of Guizhou Science and Technology [(2011)3021]~~

摘　　要：In order to investigate the possibility and efficiency of exogenous gene spread in nature and potential ecological risk of transgenic rice, as well as analyze the effect of exogenous Bt gene insertion on ecological fitness of transgenic rice plants, a experiment was carried out with three insect-resistant Bt transgenic rice cultivars Bt63, R1 and R2 and one conventional rice line 11-838 as experimental materials, the insect-resistant transgenic and non-transgenic rice plants were inter- cropped pair-wisely under high and low insect-infestation pressures, and the vegeta- tive growth, seed-setting and the resistance to rice stem borers were compared be- tween transgenic and non-transgenic lines. According to the experimental results, both the tiller number and fresh weight of Bt transgenic rice plants under low insect- infestation pressure showed no significant differences compared with the control, but the plant height, spike length and spike weight were all lower than those of non- transgenic rice plant, and Bt63 and R2 were significantly different compared with the control. On the contrary, under high insect-infestation pressure, the tiller number, spike length and spike weight of three Bt transgenic rice cultivars were significantly higher than those of the control, while the plant height showed different fitness ef- fects among various transgenic rice cultivars, which might be related to the charac- teristics of the receptive cultivars. The individual filled grain number and 1 000-grain weight of three transgenic rice cultivars showed no significant difference compared with the control under two different insect-infestation pressures, suggesting that the effect of exogenous Bt gene on seed setting was not significant. Under insect-infes- tation pressure, the resistance of three Bt transgenic rice cultivars against rice stem borer was significantly superior to non-transgenic rice, indicating that the effect of exogenous Bt gene on insect resistance of receptive plants was distinctly. Further- more, experimental[目的]提高向日葵遗传图谱的密度和实用性。[方法]以123个来源于PAC-2和RHA-266杂交的F8代重组自交系(RILs)群体为材料,利用简单序列重复(Simple sequence repeat,SSR)标记,采用MAPMARKER软件对向日葵遗传图谱进行标注。从300对SSR引物中筛选出51对有多态性的引物对(RILs)群体进行扫描。[结果]有19对引物无多态性或条带不清晰,32对引物表现多态性;共检测到35个多态性位点,分布在图谱的15条连锁群上。标记后的图谱总长度为2914.5cM,比原来的图谱增长7.5cM。标记间平均距离由9.0cM缩短为8.1cM。[结论]为进一步的向日葵遗传图谱整合和分子标记辅助选择提供参考。

关 键 词：Ecological fitness Insect-resistance Transgenic rice Insect-infestation pressure Gene flow Ecological risk 

分 类 号：S565.5[农业科学—作物学]