HPLC法测定大鼠体内8-氯腺苷及其代谢物的浓度  

Determination of 8-chloro adenosine and its metabolites in rats by HPLC

作　　者：窦桂芳[1] 楼雅卿[2] 孙莉莉 张蔚青[1] 邹安庆[1] 

机构地区：[1]北京医科大学天然药物与仿生药物国家重点实验室,北京100083 [2]北京医科大学基础医学院药理学系

出　　处：《西北药学杂志》2000年第3期99-100,共2页Northwest Pharmaceutical Journal

基　　金：为国家生命科学中心 10 35资助项目! (96 0 0 5 )

摘　　要：采用高效液相色谱法测定大鼠体内 8-氯腺苷及其代谢物 8-氯肌苷和 8-氯腺嘌呤的浓度。试样用乙腈沉淀蛋白后 ,水层进样 ,采用 YWG- C18柱 ,以甲醇 - 1 %醋酸 (含 0 .1 %四丁基溴化铵 )为流动相 ,检测波长 2 63nm。8-氯腺苷及其代谢物分离良好 ,最低检测浓度 8-氯腺苷、8-氯肌苷和 8-氯腺嘌呤分别为 0 .1 0μg/ ml,0 .50μg/ml,0 .2 5μg/ ml,日内精密度和日间精密度均在 2 .3%～ 1 6.0 % ,三种化合物回收率为 70 %～ 99%To determine the concentration of 8 chloro adenosine and its metabolites in rats with HPLC. The analytical column(250mm×4 6mm) was packed with 10μm YWG C 18 H 37 . The mobile phase was a mixture of methanol and 1% acetic acid(containing 0 1% tetrabutyl ammonium bromide)(20∶80,V/V). Detection was performed at 263nm. The 8 chloro adenosine and its two metabolites were separated very well. The minimum detectable concentrations of 8 chloro adenosine, 8 chloro adenine and 8 chloro inosine were 0 1 g/ml, 0 25 g/ml and 0 50 g/ml respectively. The precisions within day and day to day ranged from 2 3% to 16 0%. The mean recoveries ranged from 70% to 99%.

关 键 词：氯腺苷 氯腺嘌呤 高效液相色谱 药代动力学 

分 类 号：R979.1[医药卫生—药品] R969.1[医药卫生—药学]