高血压病患者血管平滑肌细胞一氧化氮合酶活性变化和精氨酸加压素对一氧化氮合成的影响  

NOS activity in vascular smooth muscle cells of hypertension patients and the effect of AVP on NO synthesis

作　　者：赵连友[1] 李宏伟[1] 李雪[1] 杨学东[1] 乔怀宇[1] 彭育红[1] 陈永清[1] 康建华[1] 

机构地区：[1]第四军医大学唐都医院心血管内科,西安710038

出　　处：《中华医学杂志》2000年第6期425-428,共4页National Medical Journal of China

基　　金：国家自然科学基金资助项目!(39670317)

摘　　要：目的　探讨高血压病患者动脉血管平滑肌细胞 (VSMCs)一氧化氮合酶 (NOS)和一氧化氮 (NO)的变化特点和精氨酸加压素 (AVP)对NO合成的影响及其在高血压发病中的意义。方法　采用复合胶原酶法分离培养 10例高血压病患者和 10例血压正常人动脉VSMCs,检测细胞培养液的NO含量和VSMCs的NOS活性 ,以及AVP对其活性的影响。结果　 (1)高血压病患者VSMCs的NO含量(0 .84nmol/ 2 .5× 10 6细胞± 0 .0 8nmol/ 2 .5× 10 6细胞 )和NOS活性 (0 .2 3nmol/min± 0 .0 8nmol/min)均明显低于血压正常对照组 (2 .48nmol/ 2 5× 10 6细胞± 0 .19nmol/ 2 .5× 10 6细胞和 0 .37nmol/min± 0 .0 6nmol/min) ,并均有非常显著性差异 (P <0 .0 1)。 (2 )在AVP干预下 ,高血压病患者VSMCs的NO含量(3.89nmol/ 2 .5× 10 6细胞± 0 .33nmol/ 2 .5× 10 6细胞 )和NOS活性 (1.81nmol/min± 0 .0 9nmol/min)均比正常对照组NO含量 (9.0 9nmol/ 2 .5× 10 6细胞± 0 75nmol/ 2 .5× 10 6细胞 )和NOS活性 (2 .80nmol/min± 0 .2 1nmol/min)降低 (P <0 .0 1) ;两组NO含量和NOS活性均随着培养时间延长和AVP浓度增加而递增 (P <0 .0 1)。 (3)培养不同时间的高血压病患者VSMCs的NO含量均随NOS活性增强而增加 ,并呈显著性正相关。结论　高血压?Objective To investigate the changes of nitric oxide(NO) and nitric oxide synthase(NOS) in vascular smooth muscle cells(VSMCs) of the essential hypertension(EH) patients, determine the effect of arginine vasopressin(AVP) on NO synthesis, and evaluate their significance in the pathogenesis of hypertension. Methods VSMCs of EH and normotensives(NT) were isolated by compound collagenase method and culturedin vitro. We detected the NO concentration and the activity of NOS in cell culture medium and determined the effects of AVP on NO synthesis and NOS activity. Results NO concentration and the activity of NOS in VSMCs of EH were 0.84 nmol/2.5×10 6 cells±0.08 nmol/2.5×10 6 cells and (0.23 nmol/min±0.08 nmol/min per 2.5×10 6 cells respectively, which were significantly lower than in VSMCs of NT (2.48 nmol/2.5×10 6 cells±0.19 nmol/2.5×10 6 cells, 0.37 nmol/min±0.06 nmol/min per 2.5×10 6 cells, respectively, both P <0.01). Under AVP induced conditions, NO concentration 3.89±0.33 nmol/2.5×10 6 cells and NOS activity 1.81 nmol/min 2.5×10 6 cells±0.09 nmol/min 2.5×10 6 cells) in VSMCs of EH were significantly lower than in VSMCs of NT (9.09 nmol/2.5×10 6±0.75 nmol/2.5×10 6 cells), 2.80 nmol/min 2.5×10 6 cells±0.21 nmol/min 2.5×10 6 cells, respectively, both P <0.01). NO concentration and NOS activity in VSMCs of both EH and NT group increased in a culture time dependent and AVP concentration dependent manner (both P <0.01). NO concentrations in VSMCs of EH in different culture time increased with the enhancement of NOS activity, and there was significant positive relevance between them (4 h r =0.940, P <0.01; 8 h r =0.993, P <0.01). Conclusion NOS NO system of EH, with attenuated responsiveness to AVP, is probably abnormal in structure or function, and is involved in the pathogenesis of essential hypertension.

关 键 词：高血压 精氨酸升压素 一氧化氮 血管平滑肌 

分 类 号：R544.1[医药卫生—心血管疾病]