胎盘造血功能的初步研究  

A preliminary study on placenta hematopoiesis

作　　者：曾凤华[1] 沈柏均[1] 王红美[1] 时庆[2] 侯怀水 马秀峰 

机构地区：[1]山东医科大学附属医院儿科,济南250012 [2]山东医科大学低温医学研究室

出　　处：《中华血液学杂志》2000年第6期291-293,共3页Chinese Journal of Hematology

基　　金：山东省科学技术委员会基金资助项目!( 1999BB1CJB5 )

摘　　要：目的　探讨胎盘在整个胎儿时期造血中的作用 ,寻找造血干 祖细胞的新来源 ,供临床移植应用。方法　分别测定脐动脉和脐静脉血中CD34+ 细胞、粒 巨噬系细胞集落 (CFU GM)及造血生长因子含量 ;胎盘绒毛膜组织切片采用HE染色和CD34 单抗免疫组化染色。结果　脐静脉血中CD34+ 、CD34+ CD38- 细胞及CFU GM含量均高于脐动脉 ,脐静脉血中含有较多的造血刺激因子 ,而造血抑制因子的含量与脐动脉相似。胎盘绒毛膜间质中可见到造血岛、网状细胞及散在分布的CD34+ 细胞。结论　胎盘是胎儿期造血器官之一。推测同时采集胎盘及脐血中的造血干细胞 ,则有可能满足成人干细胞移植的需要。Objective To study the role of placenta on fetal hematopoiesis during embryo ontogeny, so as to find a new source of hematopoietic stem/progenitor cells(HSC/HPC) for clinical transplantation. Methods Cord blood was collected seperately from cord vein and artery. CD 34 + cells were detected by FACS, CFU GM semisolid agar culture, and hematopoietic growth factors(HGFs) by ELISA. The results of HE stain and CD 34 McAb immunohistochemistry stain of placenta chorionic villi were investigated. Results The CFU GM yields and the percentage of CD 34 + and CD 34 +/CD 38 - cells in cord vein were higher than that in artery. The cord vein contained larger amount of hematopoietic stimulating factors and similar level of hematopoietic inhibitory factors as compared with that in artery. Blood island, reticular cells and scattered CD 34 + cells were found in the mesenchyme of placenta chorionic villi. Conclusion The placenta might be an another hematopoietic organ in ontogeny. It could be applied to clinical hematopoietic cell transplantation.

关 键 词：胎肋 造血功能 血细胞生成 

分 类 号：R321.5[医药卫生—人体解剖和组织胚胎学]