柿叶提取物对小鼠急性毒性和遗传毒性研究  

Experimental Study on Acute Toxicity and Genetic Toxicity of Diospyros Kaki Extract

作　　者：吴睿[1] 覃仁安[2] 尹仁杰[2] 王德勤[2] 李楚源[2] 

机构地区：[1]教育部(省部共建)中药资源科学重点实验室 广州中医药大学中药资源科学与工程研究中心,广州510006 [2]广州白云山和记黄埔中药有限公司,广州510515

出　　处：《世界科学技术-中医药现代化》2012年第6期2201-2204,共4页Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology

基　　金：科学技术部国家“十一五”科技重大专项(2009ZX09103-305):治疗缺血性中风新药BYS-HWL01的研究,负责人:李楚源

摘　　要：目的:研究柿叶提取物的急性毒性及遗传毒性。方法:对柿叶提取物进行急性经口毒性测定,并通过小鼠骨髓微核实验及小鼠精子畸形实验对其遗传毒性进行评价。结果:小鼠柿叶提取物的最大耐受剂量大于597.2 g生药/kg,为临床成人用药量的2150倍;小鼠骨髓细胞微核实验和精子畸形实验结果均显示柿叶提取物3个剂量组的微核率、精子畸形率与阳性对照组比较均有显著性差异(P<0.01)。结论:在本实验条件下,柿叶提取物对小鼠口服的毒性较小,未见明显的遗传毒性。This study was aimed to observe the acute oral toxicity and genetic toxicity of Diospyros kaki Extract. Acute oral toxicity test was performed in mice. The genetic toxicity of Diospyros kaki Extract was evaluated by bone marrow micronucleus and sperm shape abnormality test （Diospyros kaki Extract was administered to mice at the dosage of 0.00, 0.25, 5.00, and 10.0 g/kg BW）. The results showed that the acute oral maximum-tolerated dose of Diospyros kaki Extract was greater than 597.2 g/kg, which was 2150 times of the clinical dosage. There were significant statistical differences between each dosage group and positive control group （P 〈 0.01） in bone marrow micronucleus and sperm shape experiment. It was concluded that the Diospyros kaki Extract was classified as non-toxic substance. There was no genetic toxicity produced.

关 键 词：柿叶提取物 微核 急性毒性 精子畸变 

分 类 号：R123.6[医药卫生—环境卫生学]