姬松茸多糖对铅中毒大鼠胸腺Bax和Caspase-3表达的影响  

EFFECTS OF POLYSACCHARIDES FROM AGARICUS BLAZEI MURILL ON THE EXPRESSION OF BAX AND CASPASE-3 IN THYMUS OF LEAD-POISONING RATS

作　　者：王伟娟[1] 冯翠萍[1] 常明昌 程红艳[3] 张健[1] 

机构地区：[1]山西农业大学食品科学与工程学院,太谷030801 [2]山西省食用菌工程技术研究中心,太谷030801 [3]山西农业大学资源与环境学院,太谷030801

出　　处：《营养学报》2012年第6期582-585,590,共5页Acta Nutrimenta Sinica

基　　金：山西省自然科学基金(No.2010011043-1);山西农业大学博士科研启动经费项目(No.412564);山西省科技基础条件平台建设项目(No.2010091017);山西省科技攻关项目(No.021025)

摘　　要：目的了解姬松茸多糖对铅中毒大鼠胸腺Bax和caspase-3基因表达的影响。方法选择健康45 d龄SD大鼠48只,随机分为6组,雌雄各半,分别为正常对照组、多糖对照组,Pb中毒模型组和Pb中毒+低、中、高剂量多糖共6组。铅中毒模型组和低、中、高剂量组给予0.2%醋酸铅溶液,其余组给予蒸馏水,自由饮用。多糖对照组、低、中及高剂量组分别按每天每只100、50、100、200 mg/kg体重灌服姬松茸多糖液,各组灌服液体积相等,均为1ml。其它组每天每只灌胃1ml的生理盐水。饲养60d后,取大鼠胸腺,通过荧光定量PCR测定Bax和caspase-3基因表达量。结果 (1)与正常对照组相比,胸腺Bax和caspase-3的表达量,铅中毒模型组分别升高了17%和14%差异,具有统计学意义(P<0.05);多糖对照组分别降低18%和31%,差异有统计学意义(P<0.01或P<0.05)。(2)与铅中毒模型组比,三个剂量组中多糖剂量越高,表达量越低,其中高剂量组Bax和caspase-3的表达量分别降低了44%和60%,差异具有统计学意义(P<0.01)。结论姬松茸多糖对铅致大鼠凋亡基因Bax和caspase-3表达增强具有抑制作用。[营养学报,2012,34(6):582-585,590]Objective To observe the effects of polysaccharides from Agaricus blazei Murill on the expression of Bax and caspase-3 in the thymus of lead-poisoning rats.Method Forty-eight SD rats aged 45 d（female：male=1：1） were randomly divided into normal control,lead-poisoning model,polysaccharides control,lead＋low-dose polysaccharides,lead＋medium-dose polysaccharides and lead＋high-dose polysaccharides groups.Lead-poisoning group and three Lead＋polysaccharidc groups were given 0.2% lead acetate in drinking water,and the others with distilled water.Polysaccharides control group,low,medium and high dose polysaccharides groups were treated with 100,50,100 and 200 mg/kg bw polysaccharides from Agaricus blazei murill every day respectively and the others were administered the same capacity of nornral saline（0.9%）.After 60 d,the thymus was collected.The expression of Bax and caspase-3 was determined with fluorescent quantitative real-time polymerase chain reaction（FQRT-PCR）.Results（1）Compared with the normal control group,the expression of Bax and caspase-3 in lead-poisoning model group was increased respectively by 17% and 14%（P 0.05）,and decreased respectively in polysaccharides group by18% and 31%（P0.01 and P0.05）.（2） Compared with the lead-poisoning model group,the expression of Bax and Caspase-3 was decreased significantly in high dose polysaccharides group by 44% and 60%（P0.01）.Conclusion Polysaccharides from Agaricus blazei Murill can inhibit mRNA expressions of Bax and caspase-3 in lead-poisoning rats.[ACTA NUTRIMENTA SINICA,2012,34（6）：582-585,590]

关 键 词：姬松茸多糖 胸腺 凋亡基因 

分 类 号：R285.5[医药卫生—中药学]