斯氏并殖吸虫囊蚴生物学及宿主适应性研究  

Biological Characteristics of Paragonimus Skrjabini Metacercaria and Adaptation to Host River Crabs

作　　者：杨树国[1] 钟萍[1] 张光玉[1] 

机构地区：[1]湖北医药学院寄生虫学教研室,湖北省十堰市442000

出　　处：《中华全科医学》2013年第1期6-7,共2页Chinese Journal of General Practice

摘　　要：目的调查十堰地区不同月份溪蟹斯氏并殖吸虫囊蚴自然感染率,探讨理化因素对囊蚴孵出的影响,明确囊蚴生物学特性,观察囊蚴对实验动物适应性。方法定时定点捕获野外溪蟹,解剖不同月份捕获的溪蟹并计数比较其体内囊蚴感染情况,实验观察囊蚴对温度的耐受性,生理盐水、蒸馏水及胆汁孵育囊蚴,判断不同理化条件对肺吸虫后尾蚴孵出的影响。口饲囊蚴,感染后1～4月解剖小白鼠、大鼠、家兔及豚鼠,观察其在实验动物宿主体内的发育。结果全年阳性溪蟹集中分布于4～11月份,囊蚴密度6月份最高而4月份最低,溪蟹体内囊蚴对45℃及以上温度较敏感,后尾蚴在胆汁内的孵出率高于生理盐水、蒸馏水。囊蚴在大白鼠体内可发育为成虫,而小白鼠、兔、豚鼠对斯氏并殖吸虫的耐受性较差。结论斯氏并殖吸虫感染中间宿主具有季节差异性,大白鼠可作为斯氏并殖吸虫的实验终末宿主。Objective To investigate the infection rate of Paragonimus skrjabini encysted metacercaria in river crabs captured in Shiyan city in different months, explore the effects of physical and chemical factors on the hatch of metacercaria, discuss the bi- ology characteristic of Paragonimus skrjabini metacercaria, and observe the adaptation of Paragonimus skrjabini to their hosts. Methods The second intermediate hosts were anatomized and the cysts were counted in different months. The collected cysts were deal with physical and chemical factors so that the optimal conditions of incubation could be determined. The experimental animals were fed with cysts to observe the development of Paragonimus skrjabini in 1 to 4 months later. Results The positive ri- val crabs could be obtained from April to November. The infection rate of cyst in crab caught in June and April were the top and lowest one respectively. The cysts were sensitive to 45 ℃ and above temperature water. Hatchability of larvae in gall was higher than NSG and distilled water. The cysts could develop to adult in rat body, but mouse ,rabbit and guinea pig showed the different survivability to cyst. Conclusion There is significant difference in the second intermediate host infected by Paragonimus skrjabi- ni in different months. The rats could be experimental definitive host of Paragonimus skrjabini.

关 键 词：斯氏并殖吸虫 囊蚴 生物学 宿主 

分 类 号：R383.29[医药卫生—医学寄生虫学]