A new application of multiwavelets:Discrimination of marine algae taxonomic groups with emission-excitation matrix fluorescence  

作　　者：ZHANG Cui SU RongGuo ZHANG ShanShan SONG ZhiJie WANG XiuLin 

机构地区：[1]Key Laboratory of Marine Chemistry Theory and Technology,Ministry of Education [2]Ocean University of China [3]College of Information Science and Engineering,Ocean University of China

出　　处：《Science China Chemistry》2013年第1期148-158,共11页中国科学（化学英文版）

基　　金：supported by National High-Tech Research and Development Program of China (863 Program) (2009AA063005);Natural Science Foundation of Shandong Province (ZR2009EM001);Natural Science Foundation of China (40976060)

摘　　要：The 3D fluorescence discrimination of phytoplankton classes was investigated by SA4 multiwavelet,GHM multiwavelet,and coifman-2(coif2) wavelet analysis.Belonging to 35 genera of 7 major phytoplankton divisions in the inshore area of China Sea,Single species cultures of 51 phytoplankton species were employed.The second scale vector (Ca2) of SA4,Ca2 of GHM and the third scale vector (Ca3) of coif2 were selected as feature spectra by Bayesian discriminate analysis (BDA).The reference spectra were obtained via hierarchical cluster analysis (HCA).With average high correct discrimination ratios (CDRs),reference spectra were representative to phytoplankton species.For one-algae samples,the average CDRs were 95.6% at genus level and 86.7% at division level.For the laboratory mixed samples,the average CDRs (one division accounted for 25%,75% or 100% of the total biomass) were 86.6%,91.4% and 100% at division level.Moreover,the average CDRs of the dominant species (accounted for 75%) was 79.8% at genus level.Results for the in situ samples were coincided with the microscopic ones at division level with the relative contents of 54.3%-96.5%.The fluorometric discriminating technique was further tested during the cruise in Bohai Sea recently.The 3D fluorescence discrimination of phytoplankton classes was investigated by SA4 multiwavelet,GHM multiwavelet,and coifman-2（coif2） wavelet analysis.Belonging to 35 genera of 7 major phytoplankton divisions in the inshore area of China Sea,Single species cultures of 51 phytoplankton species were employed.The second scale vector （Ca2） of SA4,Ca2 of GHM and the third scale vector （Ca3） of coif2 were selected as feature spectra by Bayesian discriminate analysis （BDA）.The reference spectra were obtained via hierarchical cluster analysis （HCA）.With average high correct discrimination ratios （CDRs）,reference spectra were representative to phytoplankton species.For one-algae samples,the average CDRs were 95.6% at genus level and 86.7% at division level.For the laboratory mixed samples,the average CDRs （one division accounted for 25%,75% or 100% of the total biomass） were 86.6%,91.4% and 100% at division level.Moreover,the average CDRs of the dominant species （accounted for 75%） was 79.8% at genus level.Results for the in situ samples were coincided with the microscopic ones at division level with the relative contents of 54.3%-96.5%.The fluorometric discriminating technique was further tested during the cruise in Bohai Sea recently.

关 键 词：phytoplankton fluorescence emission-excitation matrix spectra MULTIWAVELET wavelet DISCRIMINATE 

分 类 号：Q949[生物学—植物学]