Lotus japonicus共生固氮基因的比较基因组学  

Comparative Genomics Analysis onLotus japonicus Symbiotic Nitrogen Fixation Genes

作　　者：黄世栋[1] 冀洪芳[1] 聂旋[1] 成婷婷[1] 杨艳梅[1] 王金鹏[1] 

机构地区：[1]河北联合大学理学院,河北唐山063009

出　　处：《河北联合大学学报（自然科学版）》2013年第1期92-96,102,共6页Journal of Hebei Polytechnic University:Social Science Edition

基　　金：国家自然科学基金项目(NO.31100913);河北省教育厅项目(NO.Z2011126);大学生创新性实验计划项目(X2011047)资助

摘　　要：以模式植物Lotus japonicus基因组为研究对象,首先应用了现有生物信息学软件BLAST程序对Lotus japonicus的全基因组序列进行了比较分析,鉴定两个序列之间的相似性并将其基因筛选出来;其次结合Blastp的数据结果提供的基因同源信息,和基因在染色体上的位置信息,对其全基因组进行共线性分析确定基因组中由WGD产生的重复基因有2197对,以获得Lotus japonicus多倍化发生的规模高达28.05%;再次在NCBI上初步搜索并获得与共生固氮相关的基因序列有12个同时根据获得的共生固氮相关的基因在四个禾本科物种中进行比较分析,并且对其进行了简单的系统进化分析,发现全基因组倍增可能有利于共生固氮相关的基因家族的扩增。This paper takes pattern plant Lotus japonicus genome as the research object, firstly, use of existing bioinformatics software program BLAST Lotus japonicus complete genome sequences were compared analysis,identification of two similarity between sequences and their genetic screening out; secondly, combined with Blastp data the resuits provide homologous gene information, and gene in the chromosome location information, the whole genome collinearity analysis identified in the genome of the duplicated genes have produced by WGD 2197 ,to obtain the Lotus japonicus polyploidy occurs up to the size of 28.05% ;Then in the NCBI on the initial search and access and symbi- otic nitrogen fixation genes related to sequence 12 at the same time is obtained based on symbiotic nitrogen fixation genes involved in four gramineous species for comparative analysis, and has carried on the simple phylogenetic anal- ysis, discovered the gene group multiplication may be beneficial for symbiotic nitrogen fixation genes associated with familial amplification.

关 键 词：固氮基因 比较基因组学 基因培增 系统发育树 

分 类 号：Q945.13[生物学—植物学]