2010-2012年北京地区儿童肺炎支原体流行基因型监测  

Genotype analysis of Mycoplasma pneumoniae during 2010-2012 in children from Beijing

作　　者：孙红妹[1] 薛冠华[1] 闫超[1] 冯燕玲[1] 王丽琼[1] 赵汉青[1] 李少丽[1] 曹玲[2] 

机构地区：[1]首都儿科研究所细菌研究室,北京100020 [2]首都儿科研究所附属儿童医院呼吸科

出　　处：《中华微生物学和免疫学杂志》2012年第11期939-943,共5页Chinese Journal of Microbiology and Immunology

基　　金：北京市自然科学基金（7112019）

摘　　要：目的对肺炎支原体（Mp）的暴发流行进行监测。方法采用real—timePCR法对北京地区呼吸道感染患儿进行检测；阳性标本进行M—P基因分型[M：多位点可变数目串联重复序列分析（blLVA）；P：P1-限制性片段长度多态性（P1．RFLP）]分析。结果2010年1月—2012年5月446例呼吸道感染患儿中，69例经real-timePCR检测为阳性，2010、2011及2012年上半年的感染率分别为：11．69％、15．56％及20．00％；采用我们提出的新的M-P基因分型系统，69例标本可分为11个基因型，其中M43562P1和M53562P1为2010至2011年两个主要基因型；M33562P1和M63562P1在2012年有感染上升趋势。结论在本次国际Mp流行中，北京地区2011年肺炎支原体感染率也有所增加，主要基因型为b143562P1和M53562PI；其中M43562与欧洲流行基因型相同，M53562与以色列流行基因型相同。M-P基因分型系统，可以很好地监测世界不同地区肺炎支原体流行情况。Objective To inspect the source of an outbreak with Mycoplasma pneumoniae （Mp）. Methods We carried out real-time PCR to analyze specimens collected from pediatric patients in Beijing during January 2010 to May 2012, diagnosed as pneumonia or a respiratory infection according to clinical symptoms. These positive samples were analyzed by the M-P typing system（ M：muhiple-locus variable-num- ber tandem-repeat analysis, MLVA; P： Pl-restriction fragment length polymorphism analysis, P1-RFLP ）. Results Sixty-nine specimens were tested positive to Mp by the real-time PCR in 446 specimens from pedi- atric patients. The infection rate was 11.69% , 15.56% and 20.00% respectively in 2010,2011 and the first half of 2012. According to the M-P system, 11 distinct genotypes were identified from 69 positive specimens, M43562P1 and M53562P1 were the two main genotypes that showed an increasing trend from 2010 to 2011, and M33562P1 and M63562P1 showed an increasing trend from 2011 to 2012 in China. Conclusion During this international Mp epidemic, the infection rate of Mp was also increase in Beijing in 2011, and M43562P1 and M53562P1 were the two main genotypes. Among them, M43562 were consistent with pop genotypes in Europe, and M53562 were consistent with pop genotype in Israel. The M-P system would be valuable to moni- tor the epidemic of Mp in different countries in the world.

关 键 词：肺炎支原体 基因分型 多位点可变数目串联重复序列分析 P1-限制性片段长度多态性 M-P分型系统 

分 类 号：R725.6[医药卫生—儿科]