艾滋病胃病患者胃黏膜幽门螺杆菌感染与其组织CD4和CD8细胞表达的关系研究  

CD4 and CD8 cells in gastric mucosa from AIDS patients with gastritis infected by Helicobacter pylori

作　　者：李多多[1,2] 王立巍[1,3] 郑叶[1] 曾东[1] 黄绍萍[1] 樊贞瑜[1] 须亚红[1] 陆基华[1] 殷科珊[1] 唐荣[1] 曹婕[1] 张仁芳[1] 冯艳玲[1] 程计林[1] 

机构地区：[1]上海市(复旦大学附属)公共卫生临床中心 [2]新乡医学院第一附属医院小儿内一科 [3]佳木斯大学硕士研究生

出　　处：《中华微生物学和免疫学杂志》2012年第11期949-952,共4页Chinese Journal of Microbiology and Immunology

基　　金：复旦大学上海医学院基础一临床交叉课题基金（KSF0174）;上海市（复旦大学附属）公共卫生临床中心人才引进基金（RCJJPT）

摘　　要：目的回顾分析艾滋病胃病患者胃黏膜幽门螺杆菌（且pylori）感染与患者胃黏膜CD4、CD8阳性细胞的关系。方法58例为2004-2011年在上海市公共卫生临床中心消化内科接受胃镜检查的艾滋病上腹疼痛患者，胃窦黏膜活检组织分别进行快速尿素酶和姬氏染色检测且pylori感染，HE染色病理学检查、免疫组织化学方法分析胃黏膜组织中CD4和CD8阳性细胞的表达，并应用流式细胞仪检测其外周血CD4和CD8淋巴细胞。结果研究对象Hpylori阳性26例，阴性32例；且Fylori阳性和Hpylori阴性患者胃黏膜组织CD4阳性标志细胞的表达差异没有统计学意义；然而，HFylori阳性患者胃黏膜组织中CD8阳性标志细胞率显著高于且pylori阴性患者（P〈0．05）；Hpylori阳性患者胃黏膜组织中CD8阳性标志细胞率又显著高于CD4阳性标志细胞；且pylori阳性和Hpylori阴性患者外周血CD8淋巴细胞没有显著性差异，而外周血CD4淋巴细胞绝对计数存在差别，前者高于后者（P〈0．05）。结论艾滋病患者感染HFYlori者其胃黏膜组织中CD8细胞表达水平高于Hpylori未感染者，但外周血CIM细胞数高于未感染者，其意义有待进一步探索。Objective To investigate the relationship among Helicobacter pylori（H, pylori）, CIM positive ceils and CD8 positive cells in gastric mucosa of the AIDS patients with gastritis. Methods Fifty- eight AIDS patients with upper abdominal pain were diagnosed with chronic gastritis through gastroscopy. The gastric biopsies from them were used for H. pylori detection with rapid urease test and Giemsa staining,pa- thology examination with HE staining, and immunohistochemistry analysis for CIM, CD8 positive ceils in Gas- tric mucosa. And the application of flow cytometry was for the detection of peripheral blood CIM and CD8 lymphocytes from the patients. Results H. trylori was positive in 26 cases, and negative was in 32 cases. CD8 cell expression in gastric mucosa of the AIDS patients with H. pylori positive was significantly higher than H. py/or/negative patients（ P〈0. 05 ）. There is no difference CD4 cell expression in gastric mucosa be- tween the AIDS patients with H. trylori positive and H. pylori negative patients. Moreover, CD8 positive lym- phocytes in gastric mucosa of those patients with H. pylori infection were significantly stronger than the CIM positive lymphocytes. However, the peripheral blood CD4 lymphocytes from the patients with H. pylori infec- tion were more than those from H. pylori negative patients significantly（P〈0. 05）. Conclusion The expres- sion level of CD8 cells in gastric mucosal tissues of AIDS patients with H. pylori infection were higher than those without H. pylori infection. The CD4 lymphocytes from the peripheral blood of the patients with H. pylori infection were more than those without H. pylori negative patients.

关 键 词：艾滋病 胃黏膜 CD8^+细胞 CD4^+细胞 

分 类 号：R512.91[医药卫生—内科学]