微泡造影剂对高强度聚焦超声杀伤离体棘球蚴的增强作用  

Effects of high intensity focused ultrasound combined with ultrasound contrast agent on hydatid cysts in vitro

作　　者：蔡辉[1] 张静[1,2] 叶彬[1,2] 赵毅峰[3] 郭应兴[3] 韩秀敏 张成武[3] 

机构地区：[1]重庆医科大学病原生物学教研室,重庆400016 [2]重庆医科大学分子医学与肿瘤研究中心,重庆400016 [3]青海大学医学院附属医院,西宁810001 [4]青海地方病防治研究所,西宁811602

出　　处：《中国人兽共患病学报》2012年第12期1202-1206,共5页Chinese Journal of Zoonoses

基　　金：国家自然科学基金资助项目"SAR与UCA及ABZ脂质体协同下的高强度聚焦超声杀灭细粒棘球蚴的研究"(No.30972567)~~

摘　　要：目的比较超声造影剂(ultrasound contrast agent,UCA)协同高强度聚焦超声(high intensity focused ultra-sound,HIFU)与单纯HIFU辐照对离体细粒棘球蚴包囊的杀伤效果的不同,探索UCA增强HIFU杀伤棘球蚴的方法。方法采集新鲜包囊按直径大小随机分为6个区组,每组25个,每5个包囊为1试验组随机接受以下试验:普通B超照射的空白对照,0.1mL UCA处理,单纯HIFU辐照,0.1mL UCA+HIFU辐照,0.2mL UCA+HIFU辐照。HIFU辐照后观察分析包囊灰度变化,光镜观察原头蚴形态变化并计数其死亡率。结果 HIFU辐照功率一定时,加入UCA能增加HIFU辐照包囊灰度变化和原头蚴的死亡率,并且该效果随UCA剂量的增加而加强。结论 UCA能增强HIFU对离体细粒棘球蚴杀伤效率,提高对原头蚴的杀伤效果。The aim is to evaluate the efficacy of high intensity focused ultrasound（HIFU） combined with ultrasound contrast agent（UCA） on hydatid cysts of Echinococcus granulosus in vitro,and to obtain a better method of HIFU which would be used to kill the cysts.One hundred and fifty fresh cysts were divided into 6 groups according to the diameters of cysts,and every group was received following treatments respectively： Group A（blank control） without HIFU treatment;Group B treated with 0.1 mL UCA only;Group C treated with HIFU only;Group D treated with 0.1 mL UCA and HIFU;Group E treated with 0.2 mL UCA and HIFU.During the irradiation of HIFU,the changes of ultrasonic gray scale of the cysts were recorded accordingly,and the instant death rates of protoscoleces were determined from the hydatid fluids.It was demonstrated that the changes of ultrasonic gray scale and the instant death rate of protoscoleces treated with UCA and HIFU were significant than those in the groups treated with HIFU only,or UCA only,and the changes were obvious in the groups treated with the increase of UCA.Those results suggest that UCA could enhance the killing efficacy of HIFU on hydatid cyst in vitro.

关 键 词：细粒棘球绦虫 棘球蚴 原头蚴 超声造影剂 高强度聚焦超声 

分 类 号：R532.32[医药卫生—内科学]