Neurological adaptations to hypoxia in Tibetan antelope (Pantholops hodgsonii) with a view of molecular biology of respiratory globin-neuroglobin  

作　　者：Zhen-zhong BAI Ying-zhong YANG Guo-en JIN Lan MA Ri-li Ge 

机构地区：[1]The Research Center for High Altitude Medicine,Medical School,Qinghai University

出　　处：《中国应用生理学杂志》2012年第6期555-560,共6页Chinese Journal of Applied Physiology

基　　金：supported by the"973"National Basic Research Program(2012CB51820500);National Natural Science Foundation of China(NSFC,31160219).

摘　　要：Neuroglobin(Ngb) is a respiratory protein that is preferentially expressed in brain of mouse and man.In this article,Tibetan antelope,living at altitude of 3 000～5 000 m for millions of years,was selected as the model of hypoxia-tolerant adaptation species.Using reverse transcription polymerase chain reaction(RT-PCR) and Western blot techniques,expression of Ngb gene was amplified and analyzed in antelope brain tissue.Our results showed that Ngb homology protein in Tibetan antelope was identified with more sequence similarity with cattle(96%),sheep(95%),and human(95%).We detected that there were some mutations occurred in the Open Reading Frame of Ngb in Tibetan antelope compared with sheep.Phylogenetic analysis of Ngb chain showed that it was closer to cattle than the others.This study suggests possible roles of central nervous system enriched Ngb in adaptation of Tibetan antelope to extremely high altitude.Neuroglobin（Ngb） is a respiratory protein that is preferentially expressed in brain of mouse and man.In this article,Tibetan antelope,living at altitude of 3 000～5 000 m for millions of years,was selected as the model of hypoxia-tolerant adaptation species.Using reverse transcription polymerase chain reaction（RT-PCR） and Western blot techniques,expression of Ngb gene was amplified and analyzed in antelope brain tissue.Our results showed that Ngb homology protein in Tibetan antelope was identified with more sequence similarity with cattle（96%）,sheep（95%）,and human（95%）.We detected that there were some mutations occurred in the Open Reading Frame of Ngb in Tibetan antelope compared with sheep.Phylogenetic analysis of Ngb chain showed that it was closer to cattle than the others.This study suggests possible roles of central nervous system enriched Ngb in adaptation of Tibetan antelope to extremely high altitude.

关 键 词：中枢神经系统 藏羚羊 脑红蛋白 呼吸系统 球蛋白 分子生物学 逆转录聚合酶链反应 缺氧 

分 类 号：Q426[生物学—神经生物学] Q959.842[生物学—生理学]