Effect of safflower injection on endoplasmic reticulum stress-induced apoptosis in rats with hypoxic pulmonary hypertension  

作　　者：Xiao-fang FAN Xue-rui WANG Gong-sheng YUAN Dong-hong WU Liang-gang HU Feng XUE Yong-sheng GONG 

机构地区：[1]Institute of Hypoxia Medicine,Wenzhou Medical College

出　　处：《中国应用生理学杂志》2012年第6期561-567,共7页Chinese Journal of Applied Physiology

基　　金：supported by the National Nature Science Foundation of China(30871031);the Natural Science Foundation of Zhejiang Province,China(Y2091033,206464)

摘　　要：Objective To explore the effects of safflower injection on prevention and treatment of hypoxic pulmonary hypertension and clarify the function of the endoplasmic reticulum stress apoptosis pathway during the process.Methods Thirty male SD rats were randomly grouped as normal control group,hypoxiahypercapnia group and hypoxia+safflower group.The latter two groups were put in the cabin with oxygen concentration ranged from 9% to 11% and carbon dioxide concentration from 5% to 6%.The pulmonary artery pressure and the index of right ventricular hypertrophy were determined after hypoxia exposure(8 h/d×28 d).Changes in morphology of lung tissue were observed by electron microscopy.To explore the possible mechanisms,we also detected apoptosis and apoptosis-related genes/proteins in lung tissue by TUNEL reactivity and PCR and Western blot.Results Compared with the normal control group,pulmonary artery pressure and the index of right ventricular hypertrophy in hypoxia group were 45% and 33.4% higher,respectively.Tiny blood vessel wall of lungs was thickened and edema,and proliferation of collagen fibers was obvious under the electron microscope.TUNEL staining of apoptotic cells in lung tissues showed more high brightness green fluorescence(+～++),but less green fluorescence showed in the pulmonary vascular smooth muscle cell layer,and apoptosis index(AI) value was 150% higher;gene and protein expression levels of endoplasmic reticulum stress pathway were increased.Compared with hypoxia-hypercapnia group,pulmonary artery pressure and the index of right ventricular hypertrophy in the hypoxia+safflower group were 18% and 15.6% lower,respectively;collagen fibers were decreased,and smooth muscle cells and epithelial cells were got apoptotic-like changes under the electron microscope.TUNEL staining of apoptotic cells in lung tissues showed brighter green fluorescence(++～+++);the high brightness green fluorescence showed in pulmonary vascular smooth muscle cell layer,and apoptotic index(AI) value was 40% higher;gene and protObjective To explore the effects of safflower injection on prevention and treatment of hypoxic pulmonary hypertension and clarify the function of the endoplasmic reticulum stress apoptosis pathway during the process.Methods Thirty male SD rats were randomly grouped as normal control group,hypoxiahypercapnia group and hypoxia＋safflower group.The latter two groups were put in the cabin with oxygen concentration ranged from 9% to 11% and carbon dioxide concentration from 5% to 6%.The pulmonary artery pressure and the index of right ventricular hypertrophy were determined after hypoxia exposure（8 h/d×28 d）.Changes in morphology of lung tissue were observed by electron microscopy.To explore the possible mechanisms,we also detected apoptosis and apoptosis-related genes/proteins in lung tissue by TUNEL reactivity and PCR and Western blot.Results Compared with the normal control group,pulmonary artery pressure and the index of right ventricular hypertrophy in hypoxia group were 45% and 33.4% higher,respectively.Tiny blood vessel wall of lungs was thickened and edema,and proliferation of collagen fibers was obvious under the electron microscope.TUNEL staining of apoptotic cells in lung tissues showed more high brightness green fluorescence（＋～＋＋）,but less green fluorescence showed in the pulmonary vascular smooth muscle cell layer,and apoptosis index（AI） value was 150% higher;gene and protein expression levels of endoplasmic reticulum stress pathway were increased.Compared with hypoxia-hypercapnia group,pulmonary artery pressure and the index of right ventricular hypertrophy in the hypoxia＋safflower group were 18% and 15.6% lower,respectively;collagen fibers were decreased,and smooth muscle cells and epithelial cells were got apoptotic-like changes under the electron microscope.TUNEL staining of apoptotic cells in lung tissues showed brighter green fluorescence（＋＋～＋＋＋）;the high brightness green fluorescence showed in pulmonary vascular smooth muscle cell layer,and apoptotic index（

关 键 词：细胞凋亡相关基因 肺动脉高压 内质网应激 SD大鼠 缺氧性 注射液 红花 血管平滑肌细胞 

分 类 号：S858.31[农业科学—临床兽医学] Q75[农业科学—兽医学]