Moxibustion inhibits interleukin-12 and tumor necrosis factor alpha and modulates intestinal flora in rat with ulcerative colitis  

作　　者：Xiao-Mei Wang Yuan Lu Lu-Yi Wu Shu-Guang Yu Bai-Xiao Zhao Hong-Yi Hu Huan-Gan Wu Chun-Hui Bao Hui-Rong Liu Jin-Hai Wang Yi Yao Xue-Gui Hua Hui-Ying Guo Li-Rong Shen 

机构地区：[1]Yueyang Hospital of Integrated Traditional Chinese and Western Medicine, Shanghai University of Traditional Chinese Medicine [2]Key Laboratory of Acupuncture-Moxibustion and Immunological Effects, Shanghai University of Traditional Chinese Medicine [3]Laboratory of Experimental Acupuncture of College of Acumox and Tuina, Chengdu University of Traditional Chinese Medicine, Chengdu 610075, Sichuan Province, China [4]School of Acupuncture-Moxibustion and Tuina,Beijing University of Chinese Medicine [5]Longhua Hospital, Shanghai University of Traditional Chinese Medicine

出　　处：《World Journal of Gastroenterology》2012年第46期6819-6828,共10页世界胃肠病学杂志（英文版）

基　　金：Supported by National Natural Science Foundation of China, No. 81001549;National Basic Research Program of China (973 program), No. 2009CB522900;Shanghai Health System of Outstanding Young Talent Cultivation Program, No. XYQ2011068;Shanghai Rising-Star Program, No. 10QA1406100

摘　　要：AIM: To investigate the effect of moxibustion on intestinal flora and release of interleukin-12 (IL-12) and tumor necrosis factor-α (TNF-α) from the colon in rat with ulcerative colitis (UC). METHODS: A rat model of UC was established by local stimulation of the intestine with supernatant from colonic contents harvested from human UC patients. A total of 40 male Sprague-Dawley rats were randomly divided into the following groups: normal (sham), model (UC), herb-partition moxibustion (HPM-treated), and positive control sulfasalazine (SA-treated). Rats treated with HPM received HPM at acupuncture points ST25 and RN6, once a day for 15 min, for a total of 8 d. Rats in the SA group were perfused with SA twice a day for 8 d. The colonic histopathology was observed by hematoxylin-eosin. The levels of intestinal flora, including Bifidobacterium, Lactobacillus, Escherichia coli (E. coli), and Bacteroides fragilis (B. fragilis), were tested by real-time quantitative polymerase chain reaction to detect bacterial 16S rRNA/DNA in order to determine DNA copy numbers of each specific species. Immunohistochemical assays were used to observe the expression of TNF-α and IL-12 in the rat colons. RESULTS: HPM treatment inhibited immunopathology in colonic tissues of UC rats; the general morphological score and the immunopathological score were significantly decreased in the HPM and SA groups compared with the model group [3.5 (2.0-4.0), 3.0 (1.5-3.5) vs 6.0 (5.5-7.0), P < 0.05 for the general morphological score, and 3.00 (2.00-3.50), 3.00 (2.50-3.50) vs 5.00 (4.50-5.50), P < 0.01 for the immunopathological score]. As measured by DNA copy number, we found that Bifidobacterium and Lactobacillus, which are associated with a healthy colon, were significantly higher in the HPM and SA groups than in the model group (1.395 ± 1.339, 1.461 ± 1.152 vs 0.045 ± 0.036, P < 0.01 for Bifidobacterium, and 0.395 ± 0.325, 0.851 ± 0.651 vs 0.0015 ± 0.0014, P < 0.01 for Lactobacillus). On the other hand, E. coli and B. fragilis, which are asAIM： To investigate the effect of moxibustion on intestinal flora and release of interleukin-12 （IL-12） and tumor necrosis factor-α （TNF-α） from the colon in rat with ulcerative colitis （UC）. METHODS： A rat model of UC was established by local stimulation of the intestine with supernatant from colonic contents harvested from human UC patients. A total of 40 male Sprague-Dawley rats were randomly divided into the following groups： normal （sham）, model （UC）, herb-partition moxibustion （HPM-treated）, and positive control sulfasalazine （SA-treated）. Rats treated with HPM received HPM at acupuncture points ST25 and RN6, once a day for 15 min, for a total of 8 d. Rats in the SA group were perfused with SA twice a day for 8 d. The colonic histopathology was observed by hematoxylin-eosin. The levels of intestinal flora, including Bifidobacterium, Lactobacillus, Escherichia coli （E. coli）, and Bacteroides fragilis （B. fragilis）, were tested by real-time quantitative polymerase chain reaction to detect bacterial 16S rRNA/DNA in order to determine DNA copy numbers of each specific species. Immunohistochemical assays were used to observe the expression of TNF-α and IL-12 in the rat colons. RESULTS： HPM treatment inhibited immunopathology in colonic tissues of UC rats; the general morphological score and the immunopathological score were significantly decreased in the HPM and SA groups compared with the model group [3.5 （2.0-4.0）, 3.0 （1.5-3.5） vs 6.0 （5.5-7.0）, P 〈 0.05 for the general morphological score, and 3.00 （2.00-3.50）, 3.00 （2.50-3.50） vs 5.00 （4.50-5.50）, P 〈 0.01 for the immunopathological score]. As measured by DNA copy number, we found that Bifidobacterium and Lactobacillus, which are associated with a healthy colon, were significantly higher in the HPM and SA groups than in the model group （1.395 ± 1.339, 1.461 ± 1.152 vs 0.045 ± 0.036, P 〈 0.01 for Bifidobacterium, and 0.395 ± 0.325, 0.851 ± 0.651 vs 0.0015 ± 0.0014, P 〈 0.0

关 键 词：Ulcerative colitis Herb-partition moxibustion Intestinal flora Immune regulation 

分 类 号：R245[医药卫生—针灸推拿学]