两歧双歧杆菌干预周期对IL-10基因敲除小鼠结肠上皮屏障保护作用的影响  

Influence of treatment with Bifidobacteria bifidum on gut barrier function in IL-10 knockout mice

作　　者：韩华中[1] 梁勇[2] 石忱长[2] 杨俊[2] 

机构地区：[1]苏州大学医学部,江苏省苏州市215325 [2]上海交通大学附属上海第六人民医院普外科,上海市200233

出　　处：《世界华人消化杂志》2012年第33期3203-3210,共8页World Chinese Journal of Digestology

基　　金：黎介寿院士肠道屏障研究专项基金资助项目;No.LJS-2009001~~

摘　　要：目的:探讨不同两歧双歧杆菌菌株(Bifidobacterium bifidum,B.bifidumS17)干预周期对IL-10-/-小鼠结肠上皮肠屏障功能保护作用的影响.方法:分成4组:WT组、WT+BBf(B.bifidum)组、IL-10-/-组和IL-10-/-+BBf组,周期设为2、4、6wk,5只/组,分别灌饲PBS液或B.bifidum溶液,检测结肠组织肿瘤坏死因子α(tumornecrosisfactor-α,TNF-α)、白介素1β(interleukin-1β,IL-1β)和干扰素ν(interferon-ν,INF-ν)浓度,小鼠结肠上皮通透性和TER,紧密连接蛋白的表达.结果:B.bifidum干预4wk的小鼠结肠组织TNF-α、IL-1β、INF-ν浓度,结肠黏膜上皮通透性、TER和紧密连接蛋白表达较对照组、干预2wk有显著性差异(均P<0.01),与干预6wk相比较无显著性差异.结论:B.bifidum干预4wk可检测明确的肠屏障保护作用,增加干预周期,并不能增加肠屏障保护效果.AIM： To explore the protective effect of different cycles of intervention with Bifidobacterium bifidum （B. bifidum） on colonic epithelial mucosal barrier in IL-10 knockout mice. METHODS： Female IL-10 knockout （IL-10/） mice and wild type （WT） mice were divided into four groups： WT group, WT ＋ B. biJidum （BBf） group, IL-10/ group, and IL-10/ ＋ B. bifidum group. The WT ＋ B. bifidum and IL-10-/ ＋ B. b/fi- dum groups were administered with B. bifidum by gavage at a dose of 109 cells/d per mice for 2 wk, 4 wk, and 6 wk. The other groups were giv-en phosphate buffered saline solution. The ex- pression of tumor necrosis factor （TNF）-~, inter- leukin （IL）-I~, and interferon （INF）~y in the co- lon was measured at the end of the experiment. Colonic tissues were collected for measurement of colonic epithelial permeability, transepithelial electrical resistance （TER）, and expression of tight junction protein. RESULTS： The expression of TNF-~, IL-I~, and INF-~, and tight junction protein, colonic epithe- lial permeability, and TER in mice treated with B. biJidum for 4 wk were significantly different from those in control mice and mice treated with B. bifidum for 2 wk （all P 〈 0.01）, but showed no significant differences with those in mice treated with B. bifidum for 6 wk. CONCLUSION： B. bifidum intervention for 4 wk had a significant protective effect on colonic epi- thelial mucosal barrier in IL-10 knockout mice, and prolonged intervention did not significantly improve the protective effect of B. bifidum.

关 键 词：双歧杆菌 肠屏障 紧密连接蛋白 

分 类 号：R363[医药卫生—病理学]