红花注射液对溃疡性结肠炎大鼠VEGF和bFGF表达的影响  

Effect of treatment with safflower solution on expression of VEGF and bFGF in ulcerative colitis in rats

作　　者：原皓[1] 李学兴[2] 王鹤鸣[3] 原子英[4] 赵雪曼[3] 韩雪[3] 董文华[3] 

机构地区：[1]潍坊医学院附属医院消化内科,山东省潍坊市261031 [2]安丘市人民医院消化内科,山东省安丘市262100 [3]潍坊医学院,山东省潍坊市261042 [4]青岛大学医学院,山东省青岛市266021

出　　处：《世界华人消化杂志》2012年第34期3299-3304,共6页World Chinese Journal of Digestology

摘　　要：目的:观察红花注射液(safflower solution,SS)对2,4,6-三硝基苯磺酸(2,4,6-trinitrobenzene sulfonic acid,TNBS)诱导的大鼠溃疡性结肠炎(ulcerative colitis,UC)的疗效及其对大鼠结肠组织血管内皮生长因子(vascular endothelial growth factor,VEGF)和碱性成纤维细胞生长因子(basal fibroblast growth factor,bFGF)表达的影响.方法:将30只Wistar大鼠随机分为正常对照组、模型组、红花组,每组10只.用TNBS灌肠法复制大鼠UC模型,红花组同时给予腹腔注射SS2mL/(kg·d)干预治疗,模型组与对照组给予等量生理盐水.造模10d后,收集标本.对大鼠行疾病活动指数(disease activity index,DAI)、结肠大体形态评分及组织学损伤评分,分别用免疫组织化学法及实时荧光定量PCR(real time-polymerase chain reaction,RT-PCR)技术检测大鼠结肠黏膜中VEGF、bFGF的表达.结果:与正常对照组相比,红花组、模型组DAI评分,结肠大体形态损伤评分和组织学损伤评分均显著增高(P<0.01);而与模型组相比,红花组能显著改善UC症状(P<0.05).模型组大鼠VEGF、bFGF表达水平明显高于正常组(P<0.01);与模型组相比,红花组VEGF表达显著降低(P<0.05).结论:SS能够显著改善UC大鼠结肠损伤及炎症反应,这可能与其有效下调VEGF的表达,抑制病理性血管生成有关.AIM： To evaluate the therapeutic effect of saf- flower solution on 2,4,6-trinitrobenzene sulfonic acid （TNBS）-induced ulcerative colitis in rats and to explore the underlying mechanisms by observing the expression of vascular endothe- lial growth factor （VEGF） and basal fibroblast growth factor （bFGF）.METHODS： Thirty Wistar rats were randomly divided into normal control group, model group and safflower group. Ulcerative colitis was in-duced in the safflower group and model group with TNBS by enema. Rats of the safflower group were intraperitoneally injected with saf- flower solution. After ten days, all rats were killed. Disease activity index （DAI）, colon mac- roscopic damage score （CMDS） and histologi- cal damage score were evaluated. Pathological changes in the colonic mucosa were examined by HE staining. The expression of VEGF and bFGF in the colonic mucosa was detected by real time-polymerase chain reaction （RT-PCR） and immunohistochemistry （IHC）. RESULTS： Compared to normal controls, the DAI, CMDS and histological damage scores in model rats were significantly increased （all P 〈 0.01）. Compared to the model group, the above parameters were significantly improved in the safflower group （all P 〈 0.05）. Compared to nor- mal controls, the expression levels of VEGF and bFGF in model rats were increased significantly （all P 〈 0.01）. Treatment with safflower solution significantly decreased the expression levels of VEGF mRNA and protein in ulcerative colitis （both P 〈 0.05）. CONCLUSION： Treatment with safflower solu- tion could reduce inflammatory injury in a rat model of ulcerative colitis possibly by reducing the expression of VEGF and inhibiting patho- logic angiogenesis in the colonic mucosa.

关 键 词：溃疡性结肠炎 红花注射液 血管内皮生长 因子 碱性成纤维细胞生长因子 血管生成 

分 类 号：R285.5[医药卫生—中药学]