基于RAPD标记的南苍术居群遗传多样性分析  

Analysis on genetic diversity of Atractylodes lancea populations based on RAPD marker

作　　者：郭建林[1] 葛燕芬[1] 孙小芹[1] 李密密[1] 夏冰[1] 杭悦宇[1] 

机构地区：[1]中国科学院植物研究所南京中山植物园江苏省植物迁地保护重点实验室,江苏南京210014

出　　处：《植物资源与环境学报》2012年第4期35-40,共6页Journal of Plant Resources and Environment

基　　金：江苏省自然科学基金重点项目(BK2005206)

摘　　要：采用RAPD标记技术对分布于江苏小九华山、小汤山和湖山,安徽金寨和芜湖以及湖北保康和英山的7个南苍术〔Atractylodes lancea(Thunb.)DC.〕野生居群的28个单株基因组总DNA进行PCR扩增,在此基础上分析居群的遗传多样性及遗传分化,并采用聚类分析法对居群的遗传关系进行分析。结果表明:用18条RAPD引物共扩增出193条带,其中多态性条带111条,多态性条带百分率(PPB)为57.51%;平均每条引物扩增出10.72条带,其中多态性条带6.17条。从省级水平看,安徽居群的PPB、有效等位基因数(Ne)、Nei’s基因多样性指数(H)和Shannon信息指数(I)均最低,而湖北居群的Ne、H和I均最高,但江苏居群的PPB最高;从居群水平看,湖北保康居群的PPB、Ne、H和I均最高,而安徽金寨居群均最低。7个居群的基因分化系数和基因流分别为0.206 5和1.921 5,说明7个居群总遗传变异的20.65%存在于居群间、79.35%存在于居群内。7个居群间的遗传距离为0.150 7～0.252 1,其中,安徽金寨和芜湖居群间最小(0.150 7),江苏湖山和安徽芜湖居群间最大(0.252 1)。基于遗传距离的聚类分析结果表明:7个居群可分为2组,湖北保康居群单独成组,其他6个居群聚为另一组;来自同一居群的单株均聚在一起。研究结果提示:南苍术居群间的遗传多样性较低,居群间无明显的遗传分化。By means of RAPD marker, PCR amplification of genomic total DNA of 28 individuals in seven wild populations of Atractylodes lancea （Thunb.） DC. distributing in Xiaojiuhuashan, Xiaotangshan and Hushan of Jiangsu Province, Jinzhai and Wuhu of Anhui Province and Baokang and Yingshan of Hubei Province was carried out. On this basis, genetic diversity and genetic differentiation of these populations were analyzed, and their genetic relationship was also analyzed by cluster analysis method. The results show that 193 bands are amplified by 18 RAPD primers, in which there are 111 polymorphic bands with a polymorphic band percentage （PPB） of 57.51%. Average band number amplified by one primer is 10.72 bands, in which there are 6.17 polymorphic bands. On provincial level, PPB, effective alleles number （Ne）, Nei＇ s gene diversity index （H） and Shannon＇ s information index （I） of populations of Anhui Province all are the lowest, while Ne, H and I of populations of Hubei Province all are the highest, but PPB of populations of Jiangsu Province is the highest. On population level, PPB, Ne, H and I of Baokang population of Hubei Province all are the highest, while those of Jinzhai population of Anhui Province all are the lowest. Gene differentiation coefficient and gene flow of seven populations is 0. 206 5 and 1. 912 5, respectively, indicating that 20.65% of overall genetic variation of seven populations exists among populations and 79.35% within populations. Genetic distance among seven populations is 0. 150 7-0.252 1, in which, that between populations of Jinzhai and Wuhu of Anhui Province is the smallest （0. 150 7） and that between populations of Hushan of Jiangsu Province andWuhu of Anhui Province is the biggest （0. 252 1 ）. Cluster analysis result according to genetic distance shows that seven populations can be divided into two groups, only population of Baokang of Hubei is alone in a group and the other six populations are clustered in another group. And all of individuals from the same p

关 键 词：南苍术 居群 RAPD标记 遗传多样性 聚类分析 遗传分化 

分 类 号：Q946-33[生物学—植物学] S567.213[农业科学—中草药栽培]