调Q1064nm激光联合皮肤原位再生复原技术治疗小鼠黄褐斑模型的实验研究  

Experimental Study of Q1064nm Switched Laser Combined with In Situ Skin Regenerative Restoration Technique in Treating Mouse Model of Chloasma

作　　者：谭军[1] 秦晓东[1] 欧阳华伟[1] 李波[1] 

机构地区：[1]湖南省人民医院整形激光美容科,湖南长沙410005

出　　处：《中国烧伤创疡杂志》2012年第6期423-431,共9页The Chinese Journal of Burns Wounds & Surface Ulcers

摘　　要：目的探讨调Q1064nm激光联合皮肤原位再生复原技术对小鼠黄褐斑模型黑素细胞及酪氨酸酶表达的影响。方法采用紫外线照射联合雌激素注射建立小鼠黄褐斑动物实验模型,选择调Q1064nm激光联合皮肤原位再生复原技术治疗,修复其创面,利用免疫组化方法分别检测调Q1064nm激光联合皮肤原位再生复原技术作用前后小鼠皮肤皮损区域黑色素的变化及酪氨酸酶的表达水平,并与对照组及正常组进行比较。结果与对照组相比,激光联合湿润烧伤膏(MEBO)治疗组皮损区黑色素的表达明显降低(P<0.05),酪氨酸酶的表达水平明显下降,与其他对照组相比,有显著性差异(P<0.05)。结论调Q1064nm激光干预治疗后,通过湿润烧伤膏外涂为创面创造一个再生的生理湿润环境,调节黄褐斑皮损区的局部环境,减少酪氨酸酶的表达,使黑色素的生成减少,从而抑制黄褐斑的产生。Objective To explore the effect of Q1064nm switched laser combined with in situ skin regenerative restoration technique on the expression of melanocyte and tyrosinase of mouse model of Chloasma. Methods Establish mouse Chloasma models by injecting progesterone and exposing to UVB. Select Q1064nm switched laser in combination with in situ skin regenerative restoration technique to treat and repair the wounds. Immunohistochemical technique was utilized to detect the changes of melanin and the expression of tyrosinase at the mouse skin lesions before and after the treatment, which was compared with the control group and the normal group. Results Compared with the other two groups, the expressions of melanin and tyrosinase in the experimental group decreased sharply, which had significant difference （P 〈 0. 05 respec-tively）. Conclusion The topical application of MEBO on the experimental zones after the intervention treatment of Q1064nm switched laser can maintain a moist physiological environment for wound regeneration, regulate the local environ-ment of Chloasma sites, reduce the expression of tyrosinase and the production of melanin and finally inhibit the development of Chloasma.

关 键 词：黄褐斑 调Q1064nm激光 皮肤原位再生复原技术 酪氨酸酶 实验研究 

分 类 号：R758.42[医药卫生—皮肤病学与性病学]