生长抑素在老年鼠胃窦黏膜和血浆中的表达  

Expression of somatostatin in plasma and antral mucosa in older rat

作　　者：王玉兰[1] 铁振军[1] 张泰昌[1] 

机构地区：[1]首都医科大学宣武医院中心实验室北京市老年病医疗研究中心,北京100053

出　　处：《首都医科大学学报》2012年第6期779-781,共3页Journal of Capital Medical University

基　　金：北京市卫生系统高层次卫生技术人才培养计划资助(2009-3-64)~~

摘　　要：目的研究老年鼠胃窦黏膜生长抑素含量和分布状况,探讨老化过程中生长抑素作为一种胃酸抑制因子存在的实际意义。方法选择不同年龄Wistar大鼠,雌雄不拘。青年鼠(3月龄)20只,体质量200～250 g;老年鼠(24～30月龄)15只,体质量400～450 g。动物按规定月龄取血,提取血浆样本,留取胃黏膜组织,采用放射免疫分析法,进行生长抑素测定。取胃窦新鲜组织,经冰冻切片进行生长抑素免疫组织化学染色。结果不同年龄组Wistar大鼠胃黏膜组织生长抑素具有不同的生理浓度,在老年鼠生长抑素含量呈高浓度分布,与青年鼠比较差异有统计学意义(P<0.01)。生长抑素免疫组织化学染色显示,胃窦D细胞形状不规则,胞质内布满生长抑素免疫复合物颗粒,老年鼠D细胞显色较深,其D细胞数量和免疫复合物颗粒多于青年鼠组,2者比较差异有统计学意义(P<0.05)。结论老年大鼠D细胞数量增加,生长抑素分泌量增多,与其老年抑酸调节作用增强存在重要关系。Objective To study the topography and plasma level of somatostatin of rats in order to explore the effects of somatostatin in inhibiting acid secretion in the course of ageing. Methods Different Wistar rats of different age were selected. The rats were divided into two groups： 20 young rats （age 3 months） weight 200-250 g, and 15 old rats （age 24-30 months） weight 400-450 g. The somatostatin plasma level in rats was assayed by radioimmunoassay every month, At the meantime, the frozen section of the rats sinus ventriculi were obtained to study the topography of somatostatin by immunohistochemical staining. Results The rats in different groups had different concentration of somatostatin with a high somatostatin level in old rats group. There was a significant difference between the two groups （ P〈0.01 ）. The topography of somatostatin determined by immunohistochemical staining showed that the rat gastric sinus D cell shape was irregular and full of somatostatin compound. The D cell had deeper colour. The quantity and concentration of D cell in old rats gastric sinus were more than that in young rats（ age 3 months）. The difference between the two groups was significant（P〈0.05 ）. Conclusion The quantity of D cell and the level of somatostatin in old rats gastric sinus were higher than that in young rats. These phenomena might be associated with regulation disturbance of acid secretion in older rats.

关 键 词：老年大鼠 胃黏膜 生长抑素 免疫组织化学 

分 类 号：R392[医药卫生—免疫学]