中华蟾蜍中脑视叶白介素1α、干扰素γ和肿瘤坏死因子α阳性细胞的分布  

Distribution of IL-1α- IFN-γ- and TNF-α-like immunoreactive cells in the optic lobe of Bufo gargariizans

作　　者：杨乐乐[1] 刘再群[1] 

机构地区：[1]安徽师范大学生命科学学院安徽省重要生物资源保护与利用重点实验室,生物环境与生态安全安徽省重点实验室,芜湖241000

出　　处：《解剖学杂志》2012年第6期749-752,F0003,共5页Chinese Journal of Anatomy

基　　金：安徽师范大学博士科研启动基金（160-750932）;生科院联合开放课题;安徽重要生物资源保护与利用研究安徽省重点实验室科研基金;生物环境与生态安全安徽省高校重点实验室基金

摘　　要：目的：研究中华蟾蜍中脑视叶白介素1α（IL-1α），干扰素γ（IFN-γ）和肿瘤坏死因子α（TNF-α）免疫阳性细胞的形态和分布特点，以丰富细胞因子在两栖类神经系统表达的情况，为进一步研究细胞因子对中枢神经系统的作用积累材料。方法：采用免疫组织化学SABC法和Nissl法对中华蟾蜍中脑视叶切片进行显色，光镜观察并进行统计分析处理。结果：IL-α和IFN-γ阳性细胞在中脑视叶顶盖和被盖都有很多分布，为大、中和小型细胞，而且以中、小型细胞为主，胞体为圆形和似锥形，另外顶盖带状层出现2亚层的阳性纤维；TNF-α阳性细胞只分布在中脑视叶被盖背侧区，都为中、小型细胞，胞体圆形。结论：IL-1α和IFN-γ阳性细胞广泛分布中华蟾蜍中脑视叶各区，而TNF-α阳性细胞数量较少且仅出现在视叶被盖背侧区。Objective： To investigate the distribution of IL-1α, IFN-γ- and TNF-α-like immunoreactive cells in the optic lobe of Bufo gargariizans, and get much more knowledge of cytokines expressed in the brain in amphibians to aim at providing basic information of the study on cytokines acting on the central nervous system. Methods： The immunohistochemical SABC method and Nissl staining were used to observe the distribution of cells in the optic lobe under a light microscope, and statis- tic analysis was also used. Results： There were many IL-1α- and IFN-γ- like immunoreactive cells in the whole optic lobe. They were large, middle or small sized cells, but most of them were middle and small sized cells. Their bodies were round and cone like in shape. Also, two sub-layers imunoreactive fibres were observed in the stratum zonale. TNF-α-like immuno- reactive cells with middle or small size were only distributed in the dorsal tegrnentum. Their bodies were round in shape. Conclusion： IL-1α- and IFN-γ-like immunoreactive cells were widely present in the whole optic lobe of Bufo gargariizans, but TNF-α-like immunoreactive cells were only observed in the dorsal tegmenturn.

关 键 词：白介素1Α 肿瘤坏死因子-α 干扰素-Γ 免疫组织化学 中脑视叶 中华蟾蜍 

分 类 号：R333.3[医药卫生—人体生理学]