血管一氧化氮神经调控颈总动脉血管平滑肌增殖  

Vascular nitric oxide nerves regulate smooth muscle cell proliferation of the common carotid artery

作　　者：梅麟凤[1] 黄金玉[1] 朱倩[1] 刘艳梅[1] 王旻晨[1] 吴开云[1] 

机构地区：[1]苏州大学医学部解剖学教研室,苏州215123

出　　处：《解剖学杂志》2012年第6期766-769,共4页Chinese Journal of Anatomy

摘　　要：目的：探讨血管交感神经的NO神经对血管平滑肌细胞（VSMC）增殖的影响。方法：用FeCl3诱导大鼠颈总动脉平滑肌增殖模型，实验分为假手术组，术后存活1d组、5d组，切除交感神经组和用抑制剂N-硝基-L精氨酸（LNNA）组，每组6只动物，采用荧光金（FG）逆向追踪和免疫组织化学显色标记技术，证实颈血管NO通路。H-E染色和免疫印迹检测血管平滑肌增殖变化。结果：通过FG示踪表明颈总动脉主要由颈上神经节支配，颈中、下神经节也有少量支配，NOS免疫组织化学标记证实交感神经中的NO神经支配颈血管，H-E染色可见血管损伤后有平滑肌增殖，抑制剂组和切除交感神经组增殖更明显；免疫印迹结果也显示5d组与假手术组相比细胞增殖核抗原（PCNA）表达明显上调，而LNNA组和神经切除组PCNA上调更明显。结论：支配颈总动脉的交感神经含N0神经，NO神经参与了VSMC增殖的调控。Objective： To investigate the nitric oxide （NO） pathway involved in vascular sympathetic nerves and its effect on vascular smooth muscle cell （VSMC） proliferation. Methods： VSMC proliferation of the common carotid artery was induced by FeCl3 in rats. Six groups were studied as follows： sham surgery, 1 day model, 5 day model, Nω-nitro-L-arginine （LNNA） and sympathectomy. The retrograde fluorogold tracing technique and nitric oxide synthase immunohistochemical staining were used to confirm the cervical vascular NO nerves. VSMC proliferation was determined by hematoxylin-eosin （HE） staining and Western blotting. Results： The fluorogold tracing technique demonstrated that the common carotid artery was primarily innervated by the superior cervical ganglion and partially innervated by the middle and inferior cervical ganglia. Nitric oxide synthase histochemical staining confirmed the presence of the cervical vascular NO nerves. HE staining revealed that VSMC proliferation appeared after vascular injury and that proliferation was greater in the sympathectomy and LNNA groups than in the other groups. Western blotting results showed that the expression of PCNA was significantly upregulated in the 5-day model group compared with the sham surgery group, while there was a higher amount of upregulation in the L- NNA and sympathectomy groups. Conclusion： Sympathetic nerves innervating the common carotid artery are involved in the NO pathway, which participates in the regulation of VSMC proliferation.

关 键 词：交感神经 血管 平滑肌细胞 增殖 一氧化氮合酶 荧光金示踪 N-硝基-L精氨酸 

分 类 号：R972.6[医药卫生—药品]