大鼠脑水肿脑组织谷氨酸与γ-氨基丁酸的改变及黄芩甙对其影响  

Changes of Glutamate and γ-Aminobutyric Acid Contents in Brain Tissue of Brain Edema and Effects of Baicalin on Them in Rats

作　　者：尹飞[1] 杨于嘉[1] 虞佩兰[1] 俞燕[1] 陶永光[1] 毛定安[1] 

机构地区：[1]湖南医科大学湘雅医院儿科,长沙410008

出　　处：《中国中西医结合杂志》2000年第7期524-526,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：国家自然科学基金资助!(No.39470233)

摘　　要：目的：了解大鼠感染性脑水肿脑组织谷氨酸（Ｇｌｕ）与γ－氨基丁酸（ＧＡＢＡ）的变化及黄芩甙对其影响。方法：百日咳菌液诱发大鼠感染性脑水肿及腹腔注射黄芩甙后，用高效液相色谱－荧光检测法测定大鼠脑组织匀浆中Ｇｌｕ和ＧＡＢＡ含量。结果：空白对照组（Ｃ组）、生理盐水组（ＮＳ组）、百日咳菌液组（ＰＢ组）和黄芩甙组（ＢＣ组）大鼠脑组织匀浆Ｇｌｕ含量变化不大（Ｐ＞０．０５），ＰＢ组大鼠脑组织ＧＡＢＡ含量（２．７１±０．２９）增加，ＢＣ组ＧＡＢＡ含量（４．０６±０．５２）增加更明显，与Ｃ组、ＮＳ组、ＰＢ组比较均有显著性差异（Ｐ＜０．０１）。同时ＢＣ组大鼠脑含水量（７９．８±０．４）明显下降，与ＰＢ组大鼠脑含水量（８２．２±０．３）比较有显著性差异（Ｐ＜０．０１）。结论：黄芩甙对大鼠感染性脑水肿有保护作用，其机制可能与黄芩甙能增加感染性脑水肿大鼠脑组织的ＧＡＢＡ含量有关。To observe the changes of glutamates (Glu) and γ-aminobutyric acid (GABA) contents in brain tissue of infectious brain edema, and effects of baicalin on them in rats. Methods: The high performance liquid chromatography (HPLC) was used to determine the Gin and GABA contents in homogenized brain tissue after baicalin was injected intraperitoneally on infectious brain edema induced by pertussis bacilli in rats. Results: Gin contents of homogenized brain tissue in blank control group (C), normal saline group (NS), pertussis bacilli group (PB) and baicalin group (BC) had no significant difference (P >0. 05). The concentration of GABA was more significantly increased in the BC group (4. 06 ± 0. 52) than that in the PB. group (2. 71 ± 0. 29 ) (P < 0. 05 ). Water content of brain was reduced in the BC group (79. 8 ± 0. 4) as compared with the PB group (82. 2 ± 0. 3 ) (P < 0. 01 ). Conclusion: Baicalin has a protective effect against the pertussis bacilli-induced brain edema in rats, and protective effect was correlated to the increased content of GABA in the brain tissue in rats.

关 键 词：谷氨酸 Γ-氨基丁酸 黄芩甙 脑水肿 脑组织 

分 类 号：R285[医药卫生—中药学]