中药复方双草退黄冲剂1号抗鸭乙型肝炎病毒作用的实验研究  

Experimental Study of Shuangcao Granule No. 1 on Duck Hepatitis B Virus in Ducklings

作　　者：连粤湘[1] 谭俊[1] 邓子德[2] 尹枳标[1] 吴婉芬[1] 唐小平[1] 

机构地区：[1]广州市第八人民医院传染病研究所,广州510060 [2]中山医科大学第三附属医院

出　　处：《中国中西医结合杂志》2000年第7期530-532,共3页Chinese Journal of Integrated Traditional and Western Medicine

基　　金：广东省科委重点攻关项目!(No.97027)

摘　　要：目的：研究中药复方双草退黄冲剂１号（ＳＣＧ－１）对鸭乙型肝炎病毒的体内抗病毒作用。方法：以感染鸭乙型肝炎病毒（ＤＨＢＶ）的广州麻鸭为实验动物模型。１日龄广州麻鸭感染ＤＨＢＶ，１３天后血清ＤＨＢＶ－ＤＮＡ全部阳性，给予ＳＣＧ－１治疗１４天，于感染后第１３天（为用药前），用药７、１４天及停药后３天分别采血，采用斑点杂交方法检测血清ＤＨＢＶ－ＤＮＡ。结果：ＳＣＧ－１１０ｇ／（ｋｇ· ｄ）组，用药７、１４天鸭血清ＤＨＢＶ－ＤＮＡ　ＯＤ值均明显低于给药前，有显著性差异（Ｐ＜０．０５，Ｐ＜０．０１）；ＳＣＧ－１５ｇ／（ｋｇ．ｄ）组用药１４天血清ＤＨＢＶ－ＤＮＡ ＯＤ值亦明显低于用药前（Ｐ＜０．０５），但两组停药后３天均有反跳现象。结论：ＳＣＧ－１用药期间有一定的抑制ＤＨＢＶ－ＤＮＡ复制作用。To study the in vivo anti-viral effect of a Chinese drug, composite Shuangcao Anti-Jaundice Granule No. 1 (SCG-1), on duck hepatitis B virus (DHBV). Methods: Guangzhou brown spot ducklings infected with DHBV were used as the hepatitis B animal model, for which, 1-day old ducklings were infected with DHBV. Positive DHBV-DNA in ducklings' sera were detected 13 days after the infection, and then SCG-1 was given for 14 days and DHBV-DNA was detected by dot-blot hybridization test. Results: After medicated with SCG-1 [10g/(kg·day) and 5 g/ (kg· day)] 7 days and 14 days, optic density value (OD value) of DHBV-DNA in ducklings' sera were obviously lower than that before treatment, there was significant difference. But rebound phenomenon appeared in both groups 3 days after stopping medication (P < 0. 05 ). Conclusion: SCG-1 could inhibit the replication of DHBV-DNA in Guangzhou ducklings.

关 键 词：双草退黄冲剂1号 鸭乙型炎病毒 实验研究 

分 类 号：R285[医药卫生—中药学]