日本血吸虫成虫表膜抗原的诊断效果及疗效考核价值  

EVALUATION OF SCHISTOSOMAJAPONICUM TEGUMENTAL MEMBRANE ANTIGEN (SjMAg) IN IMMUNODIAGNOSIS AND EFFICACY ASSESSMENT

作　　者：张顺科[1] 易新元[1] 舒新华[1] 李忠杰[1] 曾庆仁[1] 周金春[1] 曾宪芳[1] 

机构地区：[1]湖南医科大学寄生虫学教研室,长沙410078

出　　处：《中国寄生虫学与寄生虫病杂志》2000年第2期69-72,共4页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家"九五"科技攻关项目 !( No.96-90 6-0 4-0 4)

摘　　要：[目的 ]探讨日本血吸虫成虫表膜抗原 (Sj MAg)检测日本血吸虫病患者的特异性和敏感性及考核疗效价值。 [方法 ]采用 Sj MAg- EL ISA检测治疗前、后不同时期血吸虫病患者血清中特异性 Ig G、 Ig G4及健康人、并殖吸虫、华支睾吸虫及囊尾蚴病患者血清中的交叉抗体。 [结果 ]Sj MAg的敏感性和特异性与可溶性虫卵抗原(Sj SEA)比较 ,无显著性差异 (P>0 .0 5 ) ;用 Sj MAg- EL ISA检测治疗后 12个月患者血清中 Ig G的阴转率为80 .0 % ,显著高于 SEA- EL ISA的 43.3% ,而检测 Ig G4的阴转率为 93.3% ,显著高于 Sj SEA- EL ISA的 6 0 % ,检测治疗后 2 4个月患者血清中 Ig G和 Ig G4的阴转率分别达 92 .9%和 97.6 %。 [结论 ]Sj MAg- EL ISA检测日本血吸虫病具有与 Sj SEA- EL ISA相同的诊断价值和较高的考核疗效价值。Objective]\ To evaluate the specificity and sensitivity of SjMAg in detecting specific antibodies in sera of patients with schistosomiasis japonica and in assessing therapeutic efficacy. [Methods]\ SjMAg\|ELISA was used to determine the specific IgG and IgG\-4 in sera of patients with schistosomiasis japonica at different time points after chemotherapy. [Results]\ SjMAg\|ELISA was as sensitive and specific as SEA\|ELISA. The negative conversion rate of IgG detected by SjMAg\|ELISA 12 months after treatment was 80\^0%, being significantly higher than 43\^3% by SEA\|ELISA. The negative conversion rate of IgG\-4 detected by SjMAg\|ELISA 12 months after treatment was 93\^3%, being significantly higher than 60\^0% by SEA\|ELISA.The negative conversion rates of IgG and IgG\-4 2 yr after treatment were 92\^9% and 97\^6% respectively. [Conclusion]\ SjMAg\|ELISA is comparable to SEA\|ELISA in diagnosing schistosomiasis japonica and is more effective in assessing therapeutic efficacy.\;

关 键 词：日本血吸虫病 日本血吸虫表膜抗原 诊断 治疗 

分 类 号：R532.21[医药卫生—内科学]