猪囊尾蚴抗原-B基因cDNA编码区的克隆  

CLONING OF CYSTICERCUS CELLULOSAE AgB cDNA CODING REGION

作　　者：王庆敏[1] 戴建新[1] 张平武[1] 孙树汉[1] 

机构地区：[1]第二军医大学医学遗传学教研室,上海200433

出　　处：《中国寄生虫学与寄生虫病杂志》2000年第2期73-75,共3页Chinese Journal of Parasitology and Parasitic Diseases

摘　　要：[目的 ]扩增和克隆猪带绦虫囊尾蚴 Ag B基因的 c DNA编码区。 [方法 ]提取猪囊尾蚴总 RNA中 ,利用 RT- PCR技术扩增出 Ag B基因 c DNA编码区 ,然后将其克隆到载体 p UC118中进行序列分析。 [结果 ]PCR反应产物为单一条带 ,大小为 2 .6 kb。测序结果与澳大利亚株猪囊尾蚴 Ag B序列有 99.8%的同源性 ,二者的氨基酸序列有 99.3%的同源性。 [结论 ]成功地克隆了猪囊尾蚴 Ag B基因 c DNA编码区。Objective] To amplify and clone Cysticercus cellulosae AgB cDNA coding region. [Methods] The AgB cDNA was amplified by RT\|PCR technique from the total RNA of Cysticercus cellulosae. It was cloned into the vector pUC118 and sequenced. [Results] The PCR amplified product was a single band of 2\^6 kb in size. The sequences of AgB cDNA coding region has 99\^8% homology with that of Australian Cysticercus cellulosae, and their amino acid sequences have 99\^3% homology. [Conclusion] Cysticercus cellulosae AgB cDNA coding region has been cloned successfully.\;

关 键 词：猪带绦虫囊尾蚴 AgB基因 cDNA编码区 基因克隆 

分 类 号：R383.34[医药卫生—医学寄生虫学]