广藿香挥发油对青蒿酯钠抗伯氏疟原虫的增效作用和对抗青蒿酯钠伯氏疟原虫的逆转抗性作用  

THE SYNERGISTIC ACTION OF GUANGHUOXIANG VOLATILE OIL AND SODIUM ARTESUNATE AGAINST PLASMODIUM BERGHEI AND REVERSAL OF SA-RESISTANT PLASMODIUM BERGHEI

作　　者：刘爱如[1] 于宗渊[1] 吕丽莉[1] 隋在云[1] 

机构地区：[1]山东省中医药研究所,济南250014

出　　处：《中国寄生虫学与寄生虫病杂志》2000年第2期76-78,共3页Chinese Journal of Parasitology and Parasitic Diseases

基　　金：国家中医药管理局资助!项目 ( No.94B0 5 3 )

摘　　要：[目的 ]研究广藿香挥发油 (B)与青蒿酯钠 (SA)联合用药对伯氏疟原虫 (P.b)正常株 (N)及抗青蒿酯钠株 (R)的抗疟增效作用和对 R的逆转抗性作用。[方法 ]采用 4天抑制试验法对分别感染 N和 R小鼠进行抗疟实验 ,以两药等效剂量配伍 ,用直线回归法计算其抑制原虫 5 0 %的药物剂量 (SD5 0 )。[结果 ]B单独用药 ,N:SD5 0 =87.6 4± 19.5 8(GKD) ,R:SD5 0 =43.2 4± 7.71(GKD) ;SA单独用药 ,N:SD5 0 =0 .88± 0 .0 1(MGKD) ,R:SD5 0 =2 7.6 9± 0 .93(MGKD)。B与 SA联合用药 ,N:B的 SD5 0 =36 .89± 4.5 7(GKD) ,SA的 SD5 0 =0 .39± 0 .0 5 (MGKD) ;R的 SD5 0 =7.40± 1.30 (GKD) ,SA的 SD5 0 =4.2 1± 0 .74(MGKD)。联合用药 B对 SA的增效指数 ,N、R分别为 2 .2和 6 .6 ,B对 R的逆转抗性倍数为 6 .6。相对逆转率为 87.6 %。[结论 ]采用 B和 SA联合用药对 P.b的抑制作用具有增效作用 ,能逆转 R对 SA的抗性及延缓 N对 SA抗性的发生。Objective] To study the synergistic action of a combination of guanghuoxiang volatile oil (B) and sodium artesunate (SA) against Plasmodium berghei (P.b) and the resistance\|reversal activity against SA\|resistant P.b (P.b SA\|R). [Methods] Mice infected with P.b N or P.b R were treated with a combination of B and SA respectively by 4\|day suppressive test method and linear regression to calculate the SD\-\{50\} of B and SA for each drug alone and in combination (equally effective dose compatiblity). [Results] B alone, N:SD\-\{50\}=87\^64±19\^58(GKD),R:SD\-\{50\}=43\^24±7\^71(GKD); SA alone, N:SD\-\{50\}=0\^88±0\^01(MGKD), R:SD\-\{50\}=27\^69±0\^93(MGKD). B and SA combination, N:B SD\-\{50\}=36\^89±4\^57(GKD), SA SD\-\{50\}=0\^39±0\^05(MGKD); R:B SD\-\{50\}=7\^40±1\^30(GKD),SA SD\-\{50\}=4\^21±0\^74(MGKD). The synergistic indexes of B and SA in combination were 2\^2 for N and 6\^6 for R, respectivly. The multiple of resistance reversal of B vs SA was 6\^6. The relative reversal rate was 87\^6%. [Conclusion] A combination of B and SA may enhance the antimalarial effect against P.b and reverse the SA\|resistance of P.b and delay the occurrence of resistance to SA in N.\;

关 键 词：广藿香挥发油 青蒿酯钠 伯氏疟原虫 增效作用 

分 类 号：R978.61[医药卫生—药品]