HPLC-荧光检测法测定人血浆中佐匹克隆的浓度  

Determination of Zopiclone Concentration in Human Plasma by HPLC-fluorescent Detection

作　　者：秦超[1] 董吉[1] 缪丽燕[1] 

机构地区：[1]苏州大学附属第一医院药学部,江苏苏州215006

出　　处：《中国药房》2013年第2期125-127,共3页China Pharmacy

摘　　要：目的:建立测定人血浆中佐匹克隆浓度的方法。方法:采用液-液萃取法处理血浆后以高效液相色谱-荧光检测法进样测定,色谱柱为Kromasil C18,流动相为20mmol/L乙酸钠缓冲液(pH6.0)-乙腈-甲醇(67∶24∶9),流速为1.0ml/min,柱温为30℃,激发波长为307nm,发射波长为483nm。结果:佐匹克隆血药浓度在4～128ng/ml范围内线性关系良好(r=0.9980),定量下限为4ng/ml;方法回收率在96.3%～99.8%之间,提取回收率在75.4%～77.8%之间;日内、日间RSD均<10%。结论:本方法操作简便,专属性强、灵敏度高,可用于佐匹克隆的药动学研究。To establish a method for the determination of zopiclone concentration in human plasma. METHODS： After liquid-liquid extraction, the plasma was determined by HPLC-fluorescent detection. The determination was performed on Kromasil C18 column with mobile phase consisted of 20 mmol/L ammonium acetate buffer （pH 6.0） -acetonitrile-methanol （67： 24：9） at a flow rate of 1.0 ml/min. The column temperature was 30 ℃, and detection was performed at wavelengths of 307 and 483 nm for excitation and emission, respectively. RESULTS： The linear range of zopiclone was 4-128 ng/ml （r = 0.998 0） and the lowest limit of quantitation was 4 ng/ml. The method recovery rates were 96.3%-99.8%, and the extraction recovery rates were ranged from 75.4% to 77.8%, RSDs of inter-day and intra-day were all less than 10%. CONCLUSION： The method is simple and convenient, with high specificity and sensitivity, and suitable for pharmacokinetics study of zopiclone.

关 键 词：佐匹克隆 高效液相色谱-荧光检测法 药动学 血浆 

分 类 号：R969.1[医药卫生—药理学] R971.3[医药卫生—药学]