内生菌SZ-1的定殖力及其对姜瘟病的防效  

Colonization Ability and Effect on Ginger Bacterial Wilt of Endophyte SZ-1

作　　者：孙正祥[1] 王丰[1] 张昊[1] 邓乐[1] 周燚[1] 

机构地区：[1]长江大学农学院,湖北荆州434025

出　　处：《北方园艺》2013年第1期134-137,共4页Northern Horticulture

基　　金：湖北省教育厅中青年基金资助项目(Q2011130)

摘　　要：从无病的生姜块根中分离筛选出1株对姜瘟病菌(Ralstonia solanacearum)有较强抑制作用的内生菌SZ-1;通过利福平标记研究其在生姜根际的定殖动态;测定了菌株SZ-1发酵液对姜瘟病的防治效果。结果表明:菌株SZ-1对番茄青枯病菌(Pesudmonas solanacearumSmith)、魔芋软腐病菌(Erwinia carotovora var.carotovora)及水稻白叶枯病菌(Xanthomonas oryzae pv. oryzae)等6种病原菌均表现不同程度的抑制作用。菌株SZ-1在生姜根际数量呈先上升后下降趋势,接种50d后从根际回收的数量为4.6×105cfu/g。灌根接种30d后,姜瘟病的发病率为23.47%,病情指数为12.56,而对照发病率为89.63%,病情指数为31.52,相对防治效果为60.15%。Endophyte SZ-1which had a strong inhibition on Ralstonia solanacearum was screened from disease-free root ginger;colonization dynamics of SZ-1marked with Rifampicin on the rhizosphere of ginger was investigated;control effect of SZ-1fermentation broth on ginger bacterial wilt was illustrated.The results showed strain SZ-1had inhibitation on six pathogen including Pesudmonas solanacearum Smith,Erwinia carotovora var.carotovora,Xanthomonas oryzae pv.Oryzae etc.in varying degree.The amount of SZ-1on the rhizosphere of ginger went up first and droped later,which remained 4.6×10 5 cfu / g fifty days after inoculation.The incidence of ginger bacterial wilt was 23.47%and the disease index was 12.56thirty days after inoculation,while they were respectively 89.63%and 31.52in the control.The relative control effect was 60.15%.

关 键 词：姜瘟病 内生菌 定殖力 防治效果 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]