系统性红斑狼疮患者外周血微小RNA的表达及临床意义  

Expression of microRNA on peripheral blood cells in patients with systemic lupus erythematosus

作　　者：陈京京[1] 王丹丹[1] 周士亮[1] 吴倩[1] 冯学兵[1] 孙凌云[1] 

机构地区：[1]南京大学医学院附属鼓楼医院风湿免疫科,210008

出　　处：《中华风湿病学杂志》2013年第1期23-26,共4页Chinese Journal of Rheumatology

基　　金：国家自然科学基金（30972735）;江苏省六大人才高峰资助项目;南京市医学科技发展项目（YKK10079）

摘　　要：目的探讨系统性红斑狼疮（sLE）患者外周血骨形态发生蛋白（BMP）通路相关微小RNA（miR）-21、-155的表达水平及其临床意义。方法收集59例SLE患者和25名健康对照外周血，分离外周血单个核细胞（PBMC），Trizol法提取总RNA，逆转录过程中加polyA尾，以实时荧光定量聚合酶链反应（PCR）技术进行检测。以SLE疾病活动指数（SLEDAI）进行疾病活动性评分，根据临床症状或实验室检查结果将SLE患者分组，不同组间行Mann—Whitney秩和检验。结果与健康对照组相比，中重度活动组（SLEDAII〉8）SLE患者miR-21、-155表达水平均升高（P〈0．05），而轻度活动组（SLEDAI≤7）与健康对照组差异无统计学意义（P〉0．05）；抗双链DNA（dsDNA）（＋）SLE患者miR-21、-155表达水平显著升高（P〈0．05）；与无狼疮肾炎组相比，狼疮肾炎组SLE患者miR-21、-155表达水平升高（P〈0．05）；而miRNA表达水平与其余临床及实验室指标（血液系统异常、浆膜炎及脉管炎等）未见相关（P〉0．05）。结论中重度活动SLE患者miR．21、-155表达升高，且与抗dsDNA抗体及肾脏损伤相关，提示miR-21和-155表达异常可能在SLE疾病活动中起作用。Objective To study the expression of 2 bone morphogenetic protein （BMP） related microRNA （miR-21, miR-155） in SLE patients, and to analyze their correlation with clinical features. Methods Peripheral blood was obtained from 59 SLE patients and 25 healthy controls,and total RNA was extracted and reverse transcribed into complementary DNA （cDNA）. Real-time PCR technique was used to detect gene expression at transcriptional level. Disease activity was determined by SLEDAI score. Patients were divided into different groups based on their manifestations or antibody profiles, then comparison of miPtNA expression was carried out using Mann-Whitney test. Results The expression levels of miR-21 and miR-155 were increased in active SLE patients （SLEDAI t〉 8） as compared to those with mild disease （SLEDAI ~〈 7 ） or healthy controls （both P〈0.05）. Patients with positive anti-dsDNA or lupus nephritis had higher expression levels of miR-21 and miR-155 than those without （P〈0.05）. Conclusion The miR-21 and miR-155 expressions are elevated in active SLE patients and are associated with anti-dsDNA and renal involvement. The results suggest that these two miRNAs might play a role in the pathogenesis of SLE disease.

关 键 词：红斑狼疮 系统性 基因表达 微RNAS 自身抗体 

分 类 号：R758.62[医药卫生—皮肤病学与性病学]