由细胞质雄性不育系(CMS)配制的小麦F1杂种优势形成机理的比较蛋白质组分析  

Comparative Proteome Analysis of Heterosis in Wheat F1-hybird Bred Through Hybridization of CMS with Restoring Line

作　　者：刘晓颖[1] 段爽[1] 王振英[1] 高越[1] 彭永康[1] 

机构地区：[1]天津师范大学生命科学学院,天津300387

出　　处：《植物研究》2013年第1期51-57,共7页Bulletin of Botanical Research

基　　金：天津市科技支撑项目(10ZCKFNC00200)资助

摘　　要：分别以苗期(分蘖)、拔节期、抽穗期叶片和花粉母细胞减数分裂期、小孢子双—三核期、花粉粒时期的花药为材料,对由小麦CMS与恢复系杂交F1杂种优势形成机理作了比较蛋白质组分析。结果表明,F1杂种中有超亲、亲二型和低亲三种蛋白质表达类型出现,出现频率为亲二型>低亲>超亲。对这三种类型共17个蛋白质斑点作了质谱分析,其功能涉及DNA和蛋白质合成、能量代谢、环境防御,基因转座及光合作用等。苗期生长特性如叶鲜重、叶干重、叶片数,F1杂种倾向于双亲,没有观察到杂种优势现象,这与F1叶片中蛋白质表达多数呈亲二型相吻合。但F1中分蘖数多于双亲,因此其总鲜重、干重、总叶片数明显呈现出杂种优势,然而这种杂种优势现象与蛋白质组的变化是否有关需进一步研究。In this study,by using the leaves of seedling stage（tillering stage）,shooting stage,booting stage and anthers of PMC meiotic stage,microspore 2-3 nuclei stage,pollen grain as experimental materials,proteome approaches were applied to a comparative analysis of heterosis in wheat F1-hybrid which was bred through hybridization of CMS with restoring line.The results indicated that three types of protein expression levels,super-parents type,parent di-type,low-parents type,were observed among F1-hybrid and parents.The frequence of protein expression was showed as parent di-typelow-parents typesuper-parents type.A total of 80 protein spots were detected in F1-hybrid and parents,17 protein spots have been identified by MALDI-YOF MS.These protein spots were involved in diverse biological processes,including DNA and protein synthesis,energy metabolism,stress response,gene transposition and photosynthesis.In the F1-hybrid,at seedling stage,leaf aerial traits such as leaf fresh weight（LFW）,leaf dry weight（LDW） and leaf number,show a tendency to its parental lines when compared to parent,these results were consistent to proteome analysis results which showed most of protein spots expression in F1-hybrid exhibiting parent di-type.However,LFW,LDW,and total leaf number exhibited obviously heterosis because of tillering number（TN） increasing in F1-hybrid,it is necessary to further study the relationship between the heterosis and proteome change.

关 键 词：蛋白质组 亲二型 杂种优势 质谱 小麦 

分 类 号：S512.1[农业科学—作物学]