5-BrdU标记骨髓间充质干细胞修复椎间盘退变中的示踪研究  

Tracing study of 5-BrdU labeled MSCs in treating degenerative discs

作　　者：陈国仙 王国荣 林宗锦 李国山 罗元标 曾清东 王万明[2] 林智军[3] 刘航涛[3] 

机构地区：[1]莆田市第一医院骨科,福建莆田351100 [2]解放军南京军区福州总医院骨二科 [3]南京军区福州总医院附属第一医院骨科

出　　处：《中国中医骨伤科杂志》2013年第1期4-7,共4页Chinese Journal of Traditional Medical Traumatology & Orthopedics

摘　　要：目的:探讨5-溴脱氧尿嘧啶(5-BrdU)示踪时效及5-BrdU标记的骨髓间充质干细胞(BMSCs)在移植椎间盘后存活及细胞增殖情况。方法:分离、培养并鉴定兔BMSCs;使用模拟人后外侧入路针吸髓核法建立兔的椎间盘退变模型;注入经5-BrdU标记的骨髓间充质干细胞,在移植后第2、4、8、12周观察MSCs的增殖情况及5-BrdU示踪情况。结果:经体外标记的5-溴脱氧尿嘧啶标记率>90%;BrdU标记的BMSC移植于退变的椎间盘2周、4周、8周和12周后,在实验椎间盘内均可见BrdU染色的阳性细胞,随着时间推移,阳性细胞逐渐增多,且排列逐渐规则。结论:5-BrdU完全能够满足移植细胞的标记需要。5-BrdU标记的骨髓间充质干细胞能在所移植的椎间盘内存活及增殖。Objective：To evaluate the tracing efficacy of 5-BrdU and the proliferation of the 5-BrdU labeled bone marrow mesenchymal stem cells （MSCs） which was transplanted to treat the degenerative intervertebral discs. Methods： After the isolation,culture and identification of the rabbit BMSCs which was labeled by 5-BrdU, it was transplanted to the rabbit model established by aspiration of the nucleus pulposus. 2,4 , 8 and 12 weeks later , immunohistology was used to evaluate the proliferation of MSCs and the tracing efficacy of 5-BrdU. Result： Labeled in vitro, the labelling rate of 5-BrdU was over 90~. The labeled bone marrow mesenchymal stem cells sustained until the 12th week. The number of positive cells in- creased with time,and gradually the cells arrange in regular manner. Conclusion：5-BrdU labeled bone marrow mesenchymal stem cells can proliferate in the degenerative discs and 5-BrdU can be used to trace the transplanted MSCs completely.

关 键 词：椎间盘退变 骨髓间充质干细胞 移植 示踪 

分 类 号：R33[医药卫生—人体生理学]