栝楼毛状根培养与天花粉蛋白合成的研究  

CULTURE OF TRICHOSANTHES KIRILOWII HAIRY ROOTS AND TRICHOSANTHIN BIOSYNTHESIS

作　　者：郭志刚[1] 白桂雨[1] 张荫麟[2] 

机构地区：[1]清华大学化工系,北京100084 [2]中国医学科学院药用植物研究所

出　　处：《天然产物研究与开发》2000年第2期8-12,共5页Natural Product Research and Development

摘　　要：通过 L BA94 0 2农杆菌转化 ,诱导出了栝楼的毛状根 ;经过高压纸电泳检测甘露碱表明 ,农杆菌 Ri质粒上的 T- DNA已经进入栝楼细胞 ,并且表达合成了甘露碱 ;经实验选出了生长速度较快的 A8转化根系 ;MS培养基 ,2 5℃培养温度 ,350 lx弱光条件有利于毛状根的生物量积累 ,新鲜毛状根的天花粉蛋白含量为 0 .5～ 0 .6mg/ g。光照条件不利于天花粉蛋白的合成。The formation of hairy roots of Trichosantheskirilowii Maxim.was induced by the transformation of Agrobacterium rhizogenes LBA940 2 .The T- DNA( transfer DNA) on the Ri plasmid was affirmed to transfer into the cell of T.kirilowii by the high- voltage paper electrophoresis which could detect the mannopine's existence.The best hairy roots- A8was selected and cultivated on the MS medium,2 5℃ and35 0 lx light intensity,which were the optimum condition.On thatcondition,A8grew very well and the trichosanthin concentration of the fresh hairy roots was also comparatively high- about 0 .5～ 0 .6 mg/g,although Trichosanthin biosynthesis was inhibited in the light.

关 键 词：栝楼 毛状根 细菌转化 天花粉蛋白 中药 培养基 

分 类 号：S567.239[农业科学—中草药栽培] S188[农业科学—作物学]