魔芋葡苷聚糖(KGM)凝胶为亲和层析载体与Sepharose 4B的比较研究　Ⅱ.KGM染料亲和层析分离人血清白蛋白  

COMPARISON INVESTIGATION OF KGM GEL AND SEPHAROSE 4 B AS AFFINITY SUBSTRATES Ⅱ.Purification of HSA from human serum by dye affinity chromatography using KGM gel as substrate

作　　者：周立[1] 蒋磊[1] 郑远旗 李建吾[1] 

机构地区：[1]四川大学生命科学院,成都610064

出　　处：《天然产物研究与开发》2000年第2期23-26,共4页Natural Product Research and Development

摘　　要：将 KGM和 Sepharose4 B凝胶在相同条件下活化、偶联接上染料 CibacronBlue F3GA制成了 KGM和 Sepharose 4 B染料亲和吸附剂 ,并用来与牛血清白蛋白( BSA)作用 ,每毫升 KGM亲和吸附剂可吸附 BSA2 8mg,用 Na SCN洗脱时回收为84 .5% ,而 Sepharose 4 B梁料亲和吸附剂每毫升可吸附 BSA 15.3mg,用 Na SCN洗脱时回收为 81.7% ,并对两种凝胶的染料亲和吸附性能进行了比较。用 KGM染料亲和吸附剂分离的人血清白蛋白与人血清白蛋白标准品有同样的纯度 ,都达到了电泳纯。KGM gel and sepharose 4B were used as substrates for chromatography,Cibacron Blue F3GA as ligands to form dye affinity column.The BSA was isolated in two kinds of substrates.The 2 8mg BSA was adsorbed by 1 ml KGM gel and 1 5 .3mg BSA was adsorbed by 1 ml Sepharose 4B,The yield of BSA are 84.5 % and 81 .7% using KGM gel and sepharose4B as substrates.The human serum albumin in human serum was purified to homogeneous in one step affinity chromatography using KGM gel.

关 键 词：魔芋葡苷聚糖 染料亲和层析 血清白蛋白 载体 

分 类 号：Q512.103[生物学—生物化学] O636.11[理学—高分子化学]