不同品系小菜蛾成虫脑突触体蛋白质磷酸化的研究  

Phosphorylation of synaptosomal protein from adult brains of resistant and susceptible strains of diamond back moth

作　　者：韩招久[1] 田雨[2] 李凤良[1] 李忠英[1] 陈之浩[1] 

机构地区：[1]贵州省农业科学院植物保护研究所,贵阳550006 [2]中国科学院动物研究所,北京100080

出　　处：《昆虫学报》2000年第2期134-137,共4页Acta Entomologica Sinica

基　　金：国家自然科学基金资助项目! (39560 0 52 )

摘　　要：对小菜蛾Plutellaxylostella (L .)敏感品系、抗溴氰菊酯品系、抗杀虫双品系和抗杀螟丹品系的成虫脑突触体蛋白质磷酸化进行了研究比较。结果表明 :蛋白质磷酸化在各个品系中的表现是不一样的。cAMP和钙加钙调蛋白对不同品系小菜蛾脑蛋白质磷酸化都有不同程度的刺激作用 ;3种杀虫剂均对各品系小菜蛾的磷酸化反应有影响 ,杀虫双、杀螟丹表现为抑制 ,溴氰菊酯表现为加强。The phosphorylation of synaptosomal protein from adult brains of three resistant strains and a susceptible strain of diamond back moth, Plutella xylostella had been studied. The result showed that the level of protein phosphorylation differed between resistant and susceptible strains. Calmodulin could promoted the protein phosphorylation differently in strains studied, so was cAMP. But the susceptible strain expressed higher sensitivity. Both dimehypo and cartap inhibited the protein phosphorylation more in the susceptible strain than in dimehypo resistant and cartap resistant strains. Deltamethrin, however, activated the protein phosphorylation stronger in the susceptible strain than in deltamethrin resistant strain. The study reveals that insecticide resistance of diamond back moth might result in changes of protein phosphorylation in nervous system, leading modification of nervous regulation.

关 键 词：不菜蛾 抗药性 蛋白质磷酸化 品系 脑突触体 

分 类 号：Q966[生物学—昆虫学] S481.4[农业科学—农药学]