p28^(GANK)的表达与食管鳞癌转移的相关性  

Relationship of P28^(GANK) expression to metastasis in human esophageal squamous cell carcinoma

作　　者：赵国宏[1] 汤善宏[1] 康建琴[1] 徐光辉[1] 龚太乾[1] 赵丽娜[1] 张宏博[1] 张德新[1] 

机构地区：[1]第四军医大学西京消化病医院,陕西西安710032

出　　处：《细胞与分子免疫学杂志》2013年第1期42-45,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：国家自然科学基金(81101689)

摘　　要：目的研究p28GANK表达在食管鳞癌转移中的意义。方法免疫组化检测p28GANK在78例食管鳞癌及对应的癌旁组织中的表达水平,实时荧光定量PCR(qRT-PCR)及Western blot法检测p28GANK在两株食管癌高低转移细胞中的表达差异。结果免疫组化结果提示p28GANK在食管癌组织中阳性表达率为67.95%(53/78),显著高于对应癌旁组织阳性表达率11.42%(8/78)(P<0.01);统计分析提示,p28GANK在癌组织表达水平与患者分期、淋巴结转移、淋巴管浸润、远处转移有显著相关性(P<0.01或P<0.05);qRT-PCR及Western blot法结果提示p28GANKmRNA及蛋白在两株高转移食管癌细胞株表达水平均显著高于对应的低转移细胞系(P<0.05)。结论 p28GANK在高转移性食管癌组织及细胞系中表达显著上调。Objective To evaluate the role of p28GANK expression in the metastasis of human esophageal squamous cell carcinoma （ESCC）. Methods Immunohistochemistry was used to evaluate the expression of p28GANK in ESCC and corre- sponding adjacent tissues from 78 patients. Both Western blotting and real-time fluorescent quantitative PCR （qRT-PCR） were performed to identify the differences of p28GANK expression between highly metastatic and low metastatic ESCC cell lines. Results The positive rate of p28GANK expression in ESCC tissues was 67.95% （53/78）, which was significantly higher than that of corresponding adjacent tissues 1. 42% （8/78） （ P 〈 0. 01 ）. Statistical analyses showed that the expression of p286ANK in tumor tissues was significantly correlated with the patients＇ TNM stage （ P = 0.008）, lymph node metastasis （ P =0. 007）, lymphatic vessel invasion （ P =0.007） and distant metastasis （ P=0. 043）. qRT-PCR and Western blotting revealed that the expression of p28GANK at both mRNA and protein levels was markedly higher in the highly metastatic cells compared with matched low metastatic cells （ P 〈 0.05 ）. Conclusion The expression of p28GANK is significantly increased in ESCC tissues and cell lines with highly metastatic ability, indicating that p28GANK plays an important role in the metastasis of ESCC.

关 键 词：食管鳞癌 p28GANK 淋巴结 转移 

分 类 号：R392-33[医药卫生—免疫学] R735.1[医药卫生—基础医学]