子痫前期患者外周血树突状细胞与T细胞亚群的变化  

Changes in the subsets of dendritic cells and T cells in peripheral blood of patients with preeclampsia

作　　者：王婧[1] 苏良香[1] 朱天峰[1] 沈宓[2] 

机构地区：[1]南通市妇幼保健院,江苏南通226006 [2]南通大学医学院,江苏南通226001

出　　处：《细胞与分子免疫学杂志》2013年第1期72-75,共4页Chinese Journal of Cellular and Molecular Immunology

基　　金：江苏省南通市自然科学基金项目(S2010053)

摘　　要：目的观察子痫前期患者外周血中的树突状细胞亚群与T细胞亚群相关细胞因子的变化。方法实验组为子痫前期患者32例,对照组为未孕妇女20例,正常妊娠妇女20例。采集研究对象外周血细胞,流式细胞术检测全血细胞中髓系树突状细胞(mDC)和浆细胞样树突状细胞(pDC);分离外周血单个核细胞,经胞内细胞染色检测Th1、Th2、Th17细胞数量及Th1/Th2比值。结果子痫前期组mDC百分比(0.33 0.12)%和mDC/pDC比值(2.96 1.65)均高于正常妊娠组,二组数据有明显差异(P<0.05);pDC百分比(0.16 0.13)%较正常妊娠组(0.21 0.12)%有所下降,二组差异有统计学意义(P<0.05)。子痫前期组IFN-γ、IL-4和IL-17的百分比分别为(18.67 1.96)%、(1.88 0.51)%和(1.36 0.59)%,与正常妊娠组相比均有显著性差异(P<0.01)。子痫前期组mDC/pDC比率和Th1/Th2之间呈显著正相关(r=0.637,P<0.01);Th17表达率与pDC表达率之间呈负相关(r=-0.670,P<0.05),与mDC/pDC比率之间呈显著正相关(r=0.772,P<0.01)。结论子痫前期患者外周血中树突状细胞亚群和Th1、Th2、Th17型细胞因子异常表达,可能是患者发生免疫失衡的重要原因。Objective To investigate the immunoregulation in pathogenesis of pregnancy induced hypertension （PIH） by observing the changes in the subsets of dendritic cells （DC） and T cells in the peripheral blood of patients with preeclampsia. Methods The study included 32 preeclampsia patients, 20 normal pregnancy subjects and 20 non-pregnant healthy controls. We harvested their peripheral blood cells and counted the percentages of myeloid DC （mDC） and plasmacytoid DC （pDC） in whole blood cells, and the numbers of CD4＋ IFN-γ＋T （Thl）, CD4＋ IL-4＋T （Th2） and CD4＋ IL-17 ＋T （Th17） subsets in peripheral blood mononuclear cells by flow cytometry, and worked out the ratio of Thl to Th2. Results The percentage of mDC （0.33 ± 0.12） % and the ratio of mDC/pDC （2.96 ± 1.65） in preeclampsia patients were significantly higher than those in normal pregnancy subjects （ P 〈 0.05）. The percentage of pDC （0.16 ±0.13） % was lower than that in normal pregnancy subjects （0.21 ± 0. 12） % （ P 〈 0.05）. The percentages of IFN-γ, IL-4 and IL-17 in preeclampsia patients were （ 18.67 ± 1.96） %, （ 1.88 ± 0.51 ） % and （ 1.36 ± 0.59） %, respectively, all with statistically significant difference from those of normal late pregnancy （P〈 0.01 ）. In preeclampsia patients, mDC/pDC ratio was significantly positively correlated with Thl/Th2 （ r = 0.637, P 〈 0.01 ） and the percentage of Thl7 cells was negatively correlated with pDC ceils and positively correlated with mDC/pDC （ r = - 0.670, 0.772, respectively; P 〈 0.01 ）. Conclusion Preeclampsia patients suffered the changes in dendrit- ic cell subsets and abnormal expressions of Th1, Th2 and Th17, which might be an important reason for immune imbalance in preeclampsia patients.

关 键 词：流式细胞术 妊娠高血压 树突状细胞 TH1细胞 TH2细胞 TH17细胞 

分 类 号：R392.12[医药卫生—免疫学]