Piglets cloned from induced pluripotent stem cells  

作　　者：Nana Fan Jijun Chen Zhouchun Shang Hongwei Dou Guangzhen Ji Qingjian Zou Lu Wu Lixiazi He Fang Wang Kai Liu Na Liu Jianyong Han Qi Zhou Dengke Pan Dongshan Yang Bentian Zhao Zhen Ouyang Zhaoming Liu Yu Zhao Lin Lin Chongming Zhong Quanlei Wang Shouqi Wang Ying Xu Jing Luan Yu Liang Zhenzhen Yang Jing Li Chunxia Lu Gabor Vajta Ziyi Li Hongsheng Ouyang Huayan Wang Yong Wang Yang Yang Zhonghua Liu Hong Wei Zhidong Luan Miguel A Esteban Hongkui Deng Huanming Yang Duanqing Pei Ning Li Gang Pei Lin Liu Yutao Du Lei Xiao Liangxue Lai 

机构地区：[1]Key Laboratory of Regenerative Biology, Chinese Academy of Sciences, and Guangdong Provincial Key Laboratory of Stem Cells and Regenerative Medicine, South China Institute for Stern Cell Biology and Regenerative Medicine, Guangzhou Institutes of Biomedicine and Health, Guangzhou, Guangdong 510530, China [2]School of Life Sciences, University of Science and Technology of China, Hefei, Anhui 230027, China [3]College of Animal Sciences, Stem Cell and Developmental Biology Research Center, Zhejiang University, Hangzhou, Zhejiang 310058, China [4]Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China [5]BGI-Shenzhen, Shen- zhen, Guangdong 518083, China [6]BGI Ark Biotechnology Co., Ltd., Shenzhen, Guangdong 518083, China [7]Department of Cell Biology and Genetics, College of Life Sciences, Nankai University, Tianjin 300071, China [8]State Key Laboratory of Agrobiotechnology, College of Biological Sciences, China Agricultural University, Beijing 100193, China [9]Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China [10]Department of Gene and Cell Engineering, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing 100193, China [11]Central Queensland University, Bruce Highway, Rockhampton, QLD 4702, Australia [12]College of Animal Science and Veterinary Medicine, Jilin University, Changchun, Jilin 130062, China [13]Department of Animal Biotechnology, College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi 712100, China [14]Department of Laboratory Animal Sciences, College of Basic medicine, Third Military Medical University, Chongqing 400038, China [15]Key Laboratory of Cell Proliferation and Differentiation of the Ministry of Education, College of Life Sciences, Peking University, Beijing 100101, China [16]Center for Life Sciences, Peking University, Beijing 100101, China [17]Life Science College, North-east Agricultural University, Harbin, Heilongjiang 150030, China [

出　　处：《Cell Research》2013年第1期162-166,共5页细胞研究（英文版）

基　　金：This work is supported by grants from the Ministry of Science and Technology National Basic Research Program of China （973 program） （2009CB941001, 2011CBAOIO00, 2011CB944203, 2011 CB965101）, the National Science Fund for Distinguished Young Scholars （31025016）, the National Natural Science Foundation of China （31071293）, Maj or Program of Knowledge Innovation Plan, CAS （KSCX2-YW-R-227） and Science and Technology Planning Project of Guangdong Province, China （2011A060901019）.

摘　　要：Embryonic stem （ES） cells are powerful tools for generating genetically modified animals that can assist in advancing our knowledge of mammalian physiology and disease. Pigs provide outstanding models of human genetic diseases due to the striking similarities to human anatomy, physiology and genetics, but progress with porcine genetic engineering has been hampered by the lack of germline-competent pig ES ceils.

关 键 词：仔猪 细胞克隆 多能性 诱导式 遗传性疾病 ES细胞 转基因动物 胚胎干细胞 

分 类 号：Q785[生物学—分子生物学] S858.28[农业科学—临床兽医学]