牛肌腱胶原纤维的提取及其性能表征  

Extraction and Characterization of Collagen Fiber from Bovine Tendon

作　　者：刘萌[1,2] 李建龙[1,2] 但年华[1,2] 但卫华[1,2] 

机构地区：[1]四川大学制革清洁技术国家工程实验室,四川成都610065 [2]四川大学皮革化学与工程教育部重实验室,四川成都610065

出　　处：《中国皮革》2013年第1期19-22,共4页China Leather

基　　金：四川大学青年教师科研启动基金(2011SCU11102);成都市科技计划项目(10GGYB345SW-023)

摘　　要：以牛肌腱为原材料,通过酸-酶结合方法提取胶原纤维,采用氨基酸分析仪、红外、DSC、电泳、原子力显微镜等研究了其结构和性能。研究结果表明:胶原纤维的热变性温度为68.7℃,其氨基酸组成与I型胶原相似,纤维在1239.28、1338.67、1404.45cm-1等处具有胶原的典型红外吸收峰,SDS-PAGE电泳中只含有α、β、γ4条分离带,该原纤维的直径约为35nm。The collagen fibers were extracted from bovine tendon by acid - enzyme. The structure and performance of the col- lagen fibers were studied by IR, DSC electrophoresis and AFM. The results show that The denaturation temperature of collagen fi- bers is 68.7℃, of which the primary amino acid composition is similar to Sigma I collagen. Besides, absorption peaks of collagen are observed at 1 239. 28cm-1 ,1 338.67cm-1 ,1 404. 45cm-1, and only four separated bands of α、β、γare exhibited by SDS - PAGE Electrophoresis. Finally, the fiber diameter is 35nm.

关 键 词：胶原纤维 胶原 牛肌腱 提取 

分 类 号：TS513[轻工技术与工程—皮革化学与工程]