枯草杆菌蛋白酶基因工程的研究进展  

Advances in Gene Engineering of Subtilisin

作　　者：王凡强[1] 马美荣[1] 王正祥[1] 诸葛健[1] 

机构地区：[1]无锡轻工大学生物工程学院无锡,214036

出　　处：《生物工程进展》2000年第2期41-44,共4页Progress in Biotechnology

摘　　要：本文介绍了枯草杆菌蛋白酶 (Subtilisin)的研究现状 ,即利用定位诱变和体外重组等技术改变酶的性质 ,包括催化活性、底物特异性、稳定性、低温适应性以及酶在有机相中的性能等。对枯草杆菌蛋白酶的成功改造不仅有可观的商业价值 。The current state of study on gene engineering of subtilisin is reviewed.The characteristics of subtilisin,including catalytic activity,substrate specificity,stability,cold adaptation and performance in organic solvent system,have been successfully altered through methods such as site\|directed mutagenesis or in vitro recombination.The work to alter characteristics of subtilisin in it's performance in washing and catalysis in organic solvent,not only has considerable commercial value,but also greatly enrichs the theory of protein engineering.\;

关 键 词：枯草杆菌蛋白酶 定位诱变 体外重组 蛋白质工程 

分 类 号：Q81[生物学—生物工程] TQ93[化学工程]