猪伪狂犬病病毒新流行株的分离鉴定及抗原差异性分析  

Identification and antigenic variation of new epidemiology of pseudorabies virus from swine

作　　者：彭金美[1] 安同庆[1] 赵鸿远[1] 刘益民[1] 陈家锃[1] 冷超粮[1] 孙艳[1] 常丹[1] 田志军[1] 童光志[2] 

机构地区：[1]中国农业科学院哈尔滨兽医研究所兽医生物技术国家重点实验室/猪病研究室,黑龙江哈尔滨150001 [2]中国农业科学院上海兽医研究所,上海200241

出　　处：《中国预防兽医学报》2013年第1期1-4,共4页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家863计划(2011AA10A208);中央科研院所公益性基础科研业务费项目(ZBKJ201218)

摘　　要：2011年以来我国多个省份的规模化猪场发生了新生仔猪出现神经症状和死亡的现象,为确定其是否为猪伪狂犬病病毒(PRV)感染所引发,我们利用PCR方法从死亡的新生仔猪脑组织中扩增PRV的gE基因,发现被检猪场均存在PRV野毒感染。gE基因序列分析表明,从5个省14个猪场的病料中扩增的gE基因高度同源,与以往发表的相关序列比对显示,这些分离株均属于一个相对独立的分支。病料接种Vero细胞能够产生典型的细胞病变,将命名为PRV HeN1分离株接种小鼠能够引起瘙痒、死亡等伪狂犬病症状,并且对小鼠的LD50(102.37TCID50)显著低于经典强毒PRV双城株(103.83TCID50)。此外,中和试验结果显示,PRV Bartha k61活疫苗免疫猪仅能诱导对HeN1分离株低水平的中和抗体,而HeN1分离株能够诱导较高水平的中和抗体,并具有更强的交叉中和能力。根据本实验结果推测,近期各猪场流行的PRV可能存在一定的抗原变异。An epidemic disease characterized by neurologic symptoms and death in neonatal piglets occurred in many large scale pig farms of China's Mainland since 2011. To investigate the causative agent of the disease, the gE of pseudorabies virus （PRV） was amplified by PCR from the brain tissues of dead piglets and the results showed that PRVs were found in all detected farms. Sequence alignments of gE indicated that gE genes from 14 farms of 5 provinces in 2012 were highly homologous and they were all belong to a relatively independent sub-branch in phylogenetic analysis. In addition, the isolated PRV named HeN1 was able to cause itch and death in experimentally infected mice and the LDso of PRV HeN1 isolate was 102.37 TCIDs0 which was significantly lower than that of virulent PRV Shuangcheng strain （103.83 TCIDs0）. Furthermore, the cross-neutralization tests indicated that the serum from Bartha k61 vaccine strain inoculated pigs had low neutralizing effect to the PRV HeN1 isolate, but serum from HeN1 inoculated pigs had higher neutralizing effect to both of the viruses. In conclusion, we speculated that the prevalence of PRVs might have antigenic variation to some extent since 2011.

关 键 词：伪狂犬病病毒 鉴定 抗原差异 

分 类 号：S852.65[农业科学—基础兽医学]