肥胖基因的分离及其在大肠杆菌中的表达  

Isolation of ob Gene and Its Expression in E.coli

作　　者：邹民吉[1] 王嘉玺[1] 王利红[1] 段聚宝[1] 赵春文[1] 蔡欣[1] 

机构地区：[1]军事医学科学院基础医学研究所,北京100850

出　　处：《生物化学与生物物理进展》2000年第1期91-93,共3页Progress In Biochemistry and Biophysics

摘　　要：利用PCR技术自外周血白细胞染色体DNA中扩增获取了肥胖基因(ob基因)的外显子2和3序列.经过拼接,获得了全长的ob基因编码序列.测序结果表明,获得的序列与文献报道完全一致.利用PCR技术扩增出成熟蛋白的编码序列,克隆至表达载体pBV220中获得了表达菌株,并对表达产物进行了初步纯化,为进一步研究ob基因产物的功能与应用奠定了基础.The exon 2, 3 of ob gene were isolated from chromosome of human peripheral leucocytes by means of PCR technology. The full length coding region of ob gene was obtained by in vitro splicing with ligase. DNA sequencing confirmed that the isolated ob coding sequence is identical to that reported in the literature. The coding sequence for mature OB protein was amplified by PCR, and then cloned into an expression vector pBV220. As a result , an expression bacteria strain was obtained. The expressed product was preliminarily purified for further study.

关 键 词：肥胖基因 PCR 大肠杆菌 基因表达 减肥素 

分 类 号：R589.2[医药卫生—内分泌] Q78[医药卫生—内科学]