微柱法测定糖化血红蛋白及其临床应用  

Microcolumn Test Determination of Glycohemo globin.

作　　者：邱小麟[1] 费政芳[1] 金霆[1] 

机构地区：[1]福州市第一医院检验科,福州350009

出　　处：《生物化学与生物物理进展》2000年第1期105-106,共2页Progress In Biochemistry and Biophysics

摘　　要：糖化血红蛋白(HbA1C)作为一项良好的糖尿病(DM)患者血糖水平观测及疗效指标,已被多数临床实验室采用.但由于精确测定HbA1C的方法需要贵重的仪器设备,不适合普通实验室使用.采用微柱法(离子交换层析,BIORAD试剂)测定了80例正常人群及65例胰岛素依赖与非依赖型DM患者的全血HbA1C含量,并对该方法进行一般性评价:5个非DM与5个DM标本各重复测定10次,平均CV值为12%,HbA1C浓度为48%,89%,153%时回复实验均值为1042%.该方法不需特殊仪器设备且简便易行.实验结果表明:正常人群的HbA1C范围46%±23%,非胰岛素依赖型DM患者测定值83%～152%,胰岛素依赖型DM患者测定值8.5%～224%.住院治疗的患者HbA1C平均水平比非住院治疗者低57%.HbA1C水平的高低及维持时间的长短与患者的DM相关症状有密切关系.As a good object to observe the level of glucose in the blood,glycohemoglobin(HbA 1c )has been accepted by many clinical laboratory.The method of testing HbA 1c accurately need expensive equipment , not fit to routine laboratory. The micro column test(product of BIO RAD) was used to determine the percentage of HbA 1c in the whole blood of 80 health residents and 65 insulin dependent diabites meuitus(IDDM) and non insulin dependent diabetes (NIDDM). 5 NIDDM samples and 5 IDDM samples were assayed 10 times each in a single test run,the average value of CV was 1 2%,the concentration of HbA 1c was 4 8%,8 9%,15 3%.The average value of the repeat runs was 104 2%.The method does not require special equipment and is simple to do.The result of the test demonstrated that the overall ranges of values for the heath resident tested was 4 6%±2 3%;that for NIDDM samples was 8 3%～15 2%;the value of IDDM samples was 8 5%～22 4%. The level of HbA 1c and the time of maintain is closely associated with DM relative symptom of the patients.

关 键 词：糖化血红蛋白 糖尿病 离子交换层析 微柱法 

分 类 号：R587.105[医药卫生—内分泌]