Sox9基因在马蹄内翻足大鼠模型足踝软骨组织表达的实验研究  

The Experimental Study of Sox9 Gene Expression in Ankle Cartilage Tissue of Idiopathic Congenital Talipes Equinovarus Rat Model

作　　者：王正东[1] 颜南[2] 王效杰[1] 曾亮[1] 喻道军[3] 姜海波[1] 刘自力[1] 臧晋[1] 

机构地区：[1]沈阳医学院基础医学院解剖学教研室,辽宁沈阳110034 [2]沈阳医学院医学应用技术学院康复教研室 [3]沈阳医学院公共卫生学院检验中心

出　　处：《沈阳医学院学报》2012年第4期199-201,共3页Journal of Shenyang Medical College

基　　金：沈阳医学院科学研究基金(No.20091001)

摘　　要：目的:研究Sox9基因在软骨组织表达异常是否与马蹄内翻足发病相关。方法:制作先天性马蹄内翻足(idiopathiccongenital talipes equinovarus,ICTEV)大鼠模型,取其足踝软骨组织,用RT-PCR及免疫组化方法对Sox9基因表达量和部位与对照组进行对比分析。结果:Sox9基因在ICTEV大鼠模型足踝软骨组织的表达无论是在mRNA还是蛋白水平均比对照组高。结论:Sox9基因在ICTEV大鼠模型足踝的软骨组织表达升高可能与马蹄内翻足发病相关。Objective： To study the relation between the expression level of Sox9 gene in cartilage tissue and pathogenesis of idio- pathic congenital talipes equinovarus （ICTEV）. Method： Sox9 gene expression amount and location were analyzed by RT-PCR and immunohistochemical staining in ankle cartilage tissue of ICTEV rat model and the control group. Results： The expression level of Sox9 gene in ankle cartilage tissue of ICTEV rat model both at the mRNA or protein levels were higher than those of the control. Conclusion： The expression of Sox9 genes may be related with pathogenesis of ICTEV.

关 键 词：SOX9基因 马蹄内翻足 软骨组织 表达 

分 类 号：R682.16[医药卫生—骨科学]