锌和N-乙酰半胱氨酸对镉致人正常肝细胞HL-7702凋亡的拮抗作用研究  

The Antagonism of Zinc and N-acetyl Cysteine on Apoptosis of HL-7702 Cells Induced by Cadmium

作　　者：王雨[1] 高英伟[1] 范美[1] 伏蓉[1] 

机构地区：[1]沈阳医学院护理学院,辽宁沈阳110034

出　　处：《沈阳医学院学报》2012年第4期223-225,共3页Journal of Shenyang Medical College

基　　金：辽宁省博士启动基金资助项目(No.20091082)

摘　　要：目的:探讨氯化锌(ZnCl2)和N-乙酰半胱氨酸(N-acetyl cysteine,NAC)对镉所致人正常肝细胞HL-7702凋亡的拮抗作用。方法:体外培养的人正常肝细胞HL-7702分别以0、10、20、40、80、160μmol/L的氯化镉(CdCl2)处理24h,ZnCl2和NAC预处理组分别在各浓度的CdCl2处理前以25.0、50.0、100.0、200.0μmol/L的氯化锌和2.5、5.0、10.0、20.0 mmol/L NAC预处理24 h,MTT法测定各组HL-7702细胞的相对存活率;流式细胞术Annexin-V-PI双染法检测细胞凋亡百分率。结果:随着镉处理浓度的增加,氯化镉对细胞活力的抑制作用增强,40、80、160μmol/L的镉处理组细胞相对存活率明显降低,50μmol/L锌预处理组在CdCl2剂量为40、80μmol/L时,细胞相对存活率显著高于相应剂量的单纯镉处理组(P<0.01),10 mmol/L NAC预处理组只在40 mmol/L CdCl2组细胞相对存活率显著高于相应剂量的单纯镉处理组(P<0.05);与0μmol/L镉处理组比较,40、80、160μmol/L镉处理组HL-7702细胞的凋亡百分率显著升高(P<0.01);与40μmol/L镉处理组比较,100μmol/L锌预处理组细胞凋亡率显著降低(P<0.01);5.0、10.0、20.0 mmol/L NAC预处理可有效拮抗镉诱导的HL-7702细胞凋亡(P<0.05)。结论:锌和NAC对镉致HL-7702细胞损伤和凋亡有明显的拮抗作用。Objective： To study the antagonism of zinc chloride （ ZnCl2 ） and N-acetyl Cysteine （NAC） on apoptosis of normal human hepatoeyte HL-7702 cells induced by cadmium. Methods： Normal human hepatocyte HL-7702 ceils were incubated with 0, 10, 20, 40, 80, 160 μmol/L Cdcl2 for 24 h. The cells in groups of ZnC12 and NAC pretreatment were treated with 25.0, 50. 0, 100.0, 200.0 μmol/L ZnCl2 and2.5, 5.0, 10.0, 20.0 mmol/L NAC for24 h before incubated with 0, 10, 20, 40, 80, 160 μmol/L CdC12. Cells viability were measured by MTT. The rate of apoptosis was measured by Flowcytometry Staining of cells with phosphatidylserine （PS） -annexin- V. Results： The cells viability decreased obviously in the incubating cell with 40, 80, 160 μmol/L cadmium. The cells viability of 50. 0 μmol/L ZnC12 pretreatment group obviously increased compared with simple 40, 80 μmol/L cadmium incubating cell （P 〈 0. 01 ）. The cells viability of 10 mmol/L NAC pretreatment group increased compared with simple 40 μmol/L cadmium incubating cell （ P 〈 0. 05 ）. The apoptosis rate increased obviously in cells incubated with 40, 80, 160μmol/L cadmium compared with that in 0 p,mol/L cadmium group. The apoptosis rate in 100 μmol/L ZnC12 pretreatment decreased obriously compared with that in 40μmol/L codmium group and 5.0, 10. 0, 20. 0 mmol/L NAC pretreatment could suppress signifi- cantly cadmium-induced cell apoptosis （ P 〈 0. 05 ）. Conclusions ： ZnCl2 and NAC may suppress the apoptosis rate induced by cad- mium.

关 键 词：锌 N-乙酰半胱氨酸 镉 人 肝细胞HL-7702 凋亡 

分 类 号：R994.6[医药卫生—毒理学]