利用微卫星标记鉴定水稻的稻瘟病抗性  

Utilization of a Microsatellite Marker to Identify Rice Blast Resistance Gene in Two Segregating Populations

作　　者：李仕贵[1,2] 王玉平[1] 黎汉云[1] 周开达[1] 朱立煌[3] 

机构地区：[1]四川农业大学水稻研究所 [2]中国科学院遗传研究所北京100101 [3]中国科学院遗传研究所

出　　处：《生物工程学报》2000年第3期324-327,共4页Chinese Journal of Biotechnology

基　　金：国家自然科学基金资助项目! ( 3 980 0 10 2 )

摘　　要：应用水稻稻瘟病抗性基因 [Pi d(t) ]紧密连锁的微卫星标记RM 2 6 2对含有该抗病基因的品种地谷与感病品种江南香糯和 8987的杂交F2群体进行遗传分析和抗性鉴定 ,结果表明 ,RM2 6 2的PCR扩增物在抗、感品种之间的多态性较好 ;在 2个F2群体中 ,RM2 6 2和抗病基因间的重组率分别为 5 74%和 8 17% ,应用该标记的抗性纯合和杂合带型选择抗性植株 ,其准确率可达 98%以上。此外 。The microsatellite (SSLP) marker RM262,which is tightly linked to the blast resistance gene Pi\|d(t) in rice,provides means to conduct marker\|aided selection in a rice breeding program. The objective of this study was to investigate the ability of this marker to select the blast resistance gene,Pi\|d(t),in two crosses for rice blast resistance breeding. The products with amplified the microsatellite primers were polymorphic between the three varietes examined. To examine the power of the identified microsatellite marker in predicting the Pi\|d(t) locus,we determined the genotypes of the two F\-2 populations at the Pi\|d(t) locus by performing progeny testing for the disease respones. The results indicated an accuracy of more than 98% in identifying the resistant plants in both populations. Therefore the microsatellite marker can be utilized in marker\|assisted selection and breeding for new varieties with blast resistance.

关 键 词：水稻 稻温病 辅助育种 微卫星标记 抗病基因 

分 类 号：Q789[生物学—分子生物学]