含有FXa切割位点的抗菌肽X在大肠杆菌中的融合表达  

Fusion Expression of Cecropin X Including the Cleavage of FXa in Escherichia coli

作　　者：袁榴娣[1] 窦非[1] 梁玉璞 谢维[2] 王芳[3] 张双全[3] 戴祝英 

机构地区：[1]南京大学生物化学系南京大学医药生物技术国家重点实验室,南京210093 [2]南京铁道医学院基础医学系,南京210009 [3]南京师范大学生物系,南京210008

出　　处：《生物工程学报》2000年第3期411-414,共4页Chinese Journal of Biotechnology

基　　金：国家自然科学基金!( 3 95 70 111);铁道部科学和技术基金!( 96z0 61)资助项目

摘　　要：PCR method was used to introduce the code sequence of Factor Xa cleavage site to the 5′ end of cecropin CMIV mutant gene X,then the gene was cloned into the expression vector pGEX KG,and was highly expressed in E.coli BL21 by IPTG induction.The fusion protein was purified by affinity chromatography and was cleaved by Factor Xa.Cecropin X with antibacterial activity was obtained after purified by ion exchange chromatography.PCR method was used to introduce the code sequence of Factor Xa cleavage site to the 5′ end of cecropin CMIV mutant gene X,then the gene was cloned into the expression vector pGEX KG,and was highly expressed in E.coli BL21 by IPTG induction.The fusion protein was purified by affinity chromatography and was cleaved by Factor Xa.Cecropin X with antibacterial activity was obtained after purified by ion exchange chromatography.

关 键 词：抗菌肽X 融合表达 凝血因子FXa 抗菌活性 

分 类 号：Q786[生物学—分子生物学]